in situ hybridization

  • Open Access
    From People to <em>Panthera</em>: Natural SARS-CoV-2 Infection in Tigers and Lions at the Bronx Zoo
    Editor's Pick Research Article | Host-Microbe Biology
    From People to Panthera: Natural SARS-CoV-2 Infection in Tigers and Lions at the Bronx Zoo

    The human-animal-environment interface of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an important aspect of the coronavirus disease 2019 (COVID-19) pandemic that requires robust One Health-based investigations. Despite this, few reports describe natural infections in animals or directly link them to human infections using genomic data. In the present study, we describe the first cases of natural SARS-CoV-2 infection...

    Denise McAloose, Melissa Laverack, Leyi Wang, Mary Lea Killian, Leonardo C. Caserta, Fangfeng Yuan, Patrick K. Mitchell, Krista Queen, Matthew R. Mauldin, Brittany D. Cronk, Susan L. Bartlett, John M. Sykes, Stephanie Zec, Tracy Stokol, Karen Ingerman, Martha A. Delaney, Richard Fredrickson, Marina Ivančić, Melinda Jenkins-Moore, Katie Mozingo, Kerrie Franzen, Nichole Hines Bergeson, Laura Goodman, Haibin Wang, Ying Fang, Colleen Olmstead, Colleen McCann, Patrick Thomas, Erin Goodrich, François Elvinger, David C. Smith, Suxiang Tong, Sally Slavinski, Paul P. Calle, Karen Terio, Mia Kim Torchetti, Diego G. Diel
  • Open Access
    Quantitative Visualization of Gene Expression in Mucoid and Nonmucoid <span class="named-content genus-species" id="named-content-1">Pseudomonas aeruginosa</span> Aggregates Reveals Localized Peak Expression of Alginate in the Hypoxic Zone
    Observation | Molecular Biology and Physiology
    Quantitative Visualization of Gene Expression in Mucoid and Nonmucoid Pseudomonas aeruginosa Aggregates Reveals Localized Peak Expression of Alginate in the Hypoxic Zone

    A goal for microbial ecophysiological research is to reveal microbial activities in natural environments, including sediments, soils, or infected human tissues. Here, we report the application of the hybridization chain reaction (HCR) v3.0 to quantitatively measure microbial gene expression in situ at single-cell resolution in bacterial aggregates. Using quantitative image analysis of thousands of...

    Peter Jorth, Melanie A. Spero, J. Livingston, Dianne K. Newman
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