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Research Article | Ecological and Evolutionary ScienceBacterial Evolution in High-Osmolarity EnvironmentsFor bacteria, maintaining higher internal solute concentrations than those present in the environment allows cells to take up water. As a result, survival is challenging in high-osmolarity environments. To investigate how bacteria adapt to high-osmolarity environments, we maintained Escherichia coli in a variety of high-osmolarity solutions for hundreds of generations...
Research Article | Molecular Biology and PhysiologyTransmembrane Prolines Mediate Signal Sensing and Decoding in Bacillus subtilis DesK Histidine KinaseSignal sensing and transduction is an essential biological process for cell adaptation and survival. Histidine kinases (HK) are the sensory proteins of two-component systems that control many bacterial responses to different stimuli, like environmental changes. Here, we focused on the HK DesK from Bacillus subtilis, a paradigmatic example of a transmembrane...
Research Article | Host-Microbe BiologyThe Immune Protein Calprotectin Impacts Clostridioides difficile Metabolism through Zinc LimitationClostridioides difficile infection (CDI) is the leading cause of postantibiotic nosocomial infection. Antibiotic therapy can be successful, yet up to one-third of individuals suffer from recurrent infections. Understanding the mechanisms controlling C. difficile colonization is paramount in...