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  • Open Access
    Bacterial Evolution in High-Osmolarity Environments
    Research Article | Ecological and Evolutionary Science
    Bacterial Evolution in High-Osmolarity Environments

    For bacteria, maintaining higher internal solute concentrations than those present in the environment allows cells to take up water. As a result, survival is challenging in high-osmolarity environments. To investigate how bacteria adapt to high-osmolarity environments, we maintained Escherichia coli in a variety of high-osmolarity solutions for hundreds of generations...

    Spencer Cesar, Maya Anjur-Dietrich, Brian Yu, Ethan Li, Enrique Rojas, Norma Neff, Tim F. Cooper, Kerwyn Casey Huang
  • Open Access
    Transmembrane Prolines Mediate Signal Sensing and Decoding in <span class="named-content genus-species" id="named-content-1">Bacillus subtilis</span> DesK Histidine Kinase
    Research Article | Molecular Biology and Physiology
    Transmembrane Prolines Mediate Signal Sensing and Decoding in Bacillus subtilis DesK Histidine Kinase

    Signal sensing and transduction is an essential biological process for cell adaptation and survival. Histidine kinases (HK) are the sensory proteins of two-component systems that control many bacterial responses to different stimuli, like environmental changes. Here, we focused on the HK DesK from Bacillus subtilis, a paradigmatic example of a transmembrane...

    Pilar Fernández, Lucía Porrini, Daniela Albanesi, Luciano A. Abriata, Matteo Dal Peraro, Diego de Mendoza, María C. Mansilla
  • Open Access
    The Immune Protein Calprotectin Impacts <span class="named-content genus-species" id="named-content-1">Clostridioides difficile</span> Metabolism through Zinc Limitation
    Research Article | Host-Microbe Biology
    The Immune Protein Calprotectin Impacts Clostridioides difficile Metabolism through Zinc Limitation

    Clostridioides difficile infection (CDI) is the leading cause of postantibiotic nosocomial infection. Antibiotic therapy can be successful, yet up to one-third of individuals suffer from recurrent infections. Understanding the mechanisms controlling C. difficile colonization is paramount in...

    Christopher A. Lopez, William N. Beavers, Andy Weiss, Reece J. Knippel, Joseph P. Zackular, Walter Chazin, Eric P. Skaar
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