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Mycobacterium smegmatis

  • Open Access
    A CRISPR-Assisted Nonhomologous End-Joining Strategy for Efficient Genome Editing in <span class="named-content genus-species" id="named-content-1">Mycobacterium tuberculosis</span>
    Research Article | Molecular Biology and Physiology
    A CRISPR-Assisted Nonhomologous End-Joining Strategy for Efficient Genome Editing in Mycobacterium tuberculosis

    The global health impact of M. tuberculosis necessitates the development of new genetic tools for its manipulation, to facilitate the identification and characterization of novel drug targets and vaccine candidates. Clustered regularly interspaced short palindromic repeat (CRISPR)–CRISPR-associated protein (Cas) genome editing has proven to be a powerful genetic tool...

    Mei-Yi Yan, Si-Shang Li, Xin-Yuan Ding, Xiao-Peng Guo, Qi Jin, Yi-Cheng Sun
  • Open Access
    mRNA Degradation Rates Are Coupled to Metabolic Status in <span class="named-content genus-species" id="named-content-1">Mycobacterium smegmatis</span>
    Research Article | Molecular Biology and Physiology
    mRNA Degradation Rates Are Coupled to Metabolic Status in Mycobacterium smegmatis

    The logistics of tuberculosis therapy are difficult, requiring multiple drugs for many months. Mycobacterium tuberculosis survives in part by entering nongrowing states in which it is metabolically less active and thus less susceptible to antibiotics. Basic knowledge on how M. tuberculosis survives...

    Diego A. Vargas-Blanco, Ying Zhou, L. Gregory Zamalloa, Tim Antonelli, Scarlet S. Shell
  • Open Access
    ORBIT: a New Paradigm for Genetic Engineering of Mycobacterial Chromosomes
    Research Article | Molecular Biology and Physiology
    ORBIT: a New Paradigm for Genetic Engineering of Mycobacterial Chromosomes

    We sought to develop a system that could increase the usefulness of oligonucleotide-mediated recombineering of bacterial chromosomes by expanding the types of modifications generated by an oligonucleotide (i.e., insertions and deletions) and by making recombinant formation a selectable event. This paper describes such a system for use in M. smegmatis and...

    Kenan C. Murphy, Samantha J. Nelson, Subhalaxmi Nambi, Kadamba Papavinasasundaram, Christina E. Baer, Christopher M. Sassetti
  • Open Access
    Research Article
    HupB Is a Bacterial Nucleoid-Associated Protein with an Indispensable Eukaryotic-Like Tail
    Joanna Hołówka, Damian Trojanowski, Katarzyna Ginda, Bartosz Wojtaś, Bartłomiej Gielniewski, Dagmara Jakimowicz, Jolanta Zakrzewska-Czerwińska
  • Open Access
    Research Article
    A Novel Calcium Uptake Transporter of Uncharacterized P-Type ATPase Family Supplies Calcium for Cell Surface Integrity in Mycobacterium smegmatis
    Hemant Kumar Gupta, Shruti Shrivastava, Rakesh Sharma
  • Open Access
    Research Article
    Distinct Spatiotemporal Dynamics of Peptidoglycan Synthesis between Mycobacterium smegmatis and Mycobacterium tuberculosis
    Helene Botella, Guangli Yang, Ouathek Ouerfelli, Sabine Ehrt, Carl F. Nathan, Julien Vaubourgeix
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