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genome editing

  • Open Access
    A CRISPR-Assisted Nonhomologous End-Joining Strategy for Efficient Genome Editing in <span class="named-content genus-species" id="named-content-1">Mycobacterium tuberculosis</span>
    Research Article | Molecular Biology and Physiology
    A CRISPR-Assisted Nonhomologous End-Joining Strategy for Efficient Genome Editing in Mycobacterium tuberculosis

    The global health impact of M. tuberculosis necessitates the development of new genetic tools for its manipulation, to facilitate the identification and characterization of novel drug targets and vaccine candidates. Clustered regularly interspaced short palindromic repeat (CRISPR)–CRISPR-associated protein (Cas) genome editing has proven to be a powerful genetic tool...

    Mei-Yi Yan, Si-Shang Li, Xin-Yuan Ding, Xiao-Peng Guo, Qi Jin, Yi-Cheng Sun
  • Open Access
    The Expanded Universe of Prokaryotic Argonaute Proteins
    Research Article | Molecular Biology and Physiology
    The Expanded Universe of Prokaryotic Argonaute Proteins

    The eukaryotic Ago proteins and the RNA interference pathways they are involved in are widely used as a powerful tool in research and as potential therapeutics. In contrast, the properties and functions of prokaryotic Ago (pAgo) proteins have remained poorly understood. Understanding the diversity and functions of pAgos holds a huge potential for discovery of new cellular pathways and novel tools for genome manipulations. Only few pAgos...

    Sergei Ryazansky, Andrey Kulbachinskiy, Alexei A. Aravin
  • Open Access
    Research Article
    An Attenuated CRISPR-Cas System in Enterococcus faecalis Permits DNA Acquisition
    Karthik Hullahalli, Marinelle Rodrigues, Uyen Thy Nguyen, Kelli Palmer
  • Open Access
    Research Article
    Efficient and Scalable Precision Genome Editing in Staphylococcus aureus through Conditional Recombineering and CRISPR/Cas9-Mediated Counterselection
    Kelsi Penewit, Elizabeth A. Holmes, Kathryn McLean, Mingxin Ren, Adam Waalkes, Stephen J. Salipante
  • Open Access
    Research Article
    Rapid, Selection-Free, High-Efficiency Genome Editing in Protozoan Parasites Using CRISPR-Cas9 Ribonucleoproteins

    Protozoan parasites remain some of the highest-impact human and animal pathogens, with very limited treatment and prevention options. The development of improved therapeutics and vaccines depends on a better understanding of the unique biology of these organisms, and understanding their biology, in turn, requires the ability to track and manipulate the products of genes. In this work, we describe new methods that are available to...

    Lia Carolina Soares Medeiros, Lilith South, Duo Peng, Juan M. Bustamante, Wei Wang, Molly Bunkofske, Natasha Perumal, Fernando Sanchez-Valdez, Rick L. Tarleton
  • Open Access
    Research Article
    A Variant PfCRT Isoform Can Contribute to Plasmodium falciparum Resistance to the First-Line Partner Drug Piperaquine
    Satish K. Dhingra, Devasha Redhi, Jill M. Combrinck, Tomas Yeo, John Okombo, Philipp P. Henrich, Annie N. Cowell, Purva Gupta, Matthew L. Stegman, Jonathan M. Hoke, Roland A. Cooper, Elizabeth Winzeler, Sachel Mok, Timothy J. Egan, David A. Fidock
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