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fluorescence microscopy

  • Open Access
    The Nucleoid Occlusion Protein SlmA Binds to Lipid Membranes
    Editor's Pick Research Article | Molecular Biology and Physiology
    The Nucleoid Occlusion Protein SlmA Binds to Lipid Membranes

    Successful bacterial proliferation relies on the spatial and temporal precision of cytokinesis and its regulation by systems that protect the integrity of the nucleoid. In Escherichia coli, one of these protectors is SlmA protein, which binds to specific DNA sites around the nucleoid and helps to shield the nucleoid from inappropriate bisection by the cell division...

    Miguel Ángel Robles-Ramos, William Margolin, Marta Sobrinos-Sanguino, Carlos Alfonso, Germán Rivas, Begoña Monterroso, Silvia Zorrilla
  • Open Access
    Clustering as a Means To Control Nitrate Respiration Efficiency and Toxicity in <span class="named-content genus-species" id="named-content-1">Escherichia coli</span>
    Research Article | Molecular Biology and Physiology
    Clustering as a Means To Control Nitrate Respiration Efficiency and Toxicity in Escherichia coli

    Most bacteria rely on the redox activity of respiratory complexes embedded in the cytoplasmic membrane to gain energy in the form of ATP and of an electrochemical gradient established across the membrane. Nevertheless, production of harmful and toxic nitric oxide by actively growing bacteria as either an intermediate or side-product of nitrate respiration challenges how homeostasis control is exerted. Here, we show that components of...

    Suzy Bulot, Stéphane Audebert, Laetitia Pieulle, Farida Seduk, Emilie Baudelet, Leon Espinosa, Marie-Camille Pizay, Luc Camoin, Axel Magalon
  • Open Access
    Segregation but Not Replication of the <em>Pseudomonas aeruginosa</em> Chromosome Terminates at <em>Dif</em>
    Research Article | Molecular Biology and Physiology
    Segregation but Not Replication of the Pseudomonas aeruginosa Chromosome Terminates at Dif

    Segregation of genetic information is a central event in cellular life. In bacteria, chromosome segregation occurs concurrently with replication, sequentially along the two arms from oriC to dif. How the two processes are coordinated is unknown. We explored here chromosome segregation in an opportunistic human pathogen, Pseudomonas aeruginosa, using...

    Bijit K. Bhowmik, April L. Clevenger, Hang Zhao, Valentin V. Rybenkov
  • Open Access
    Editor's Pick Research Article
    Colocalization and Disposition of Cellulosomes in Clostridium clariflavum as Revealed by Correlative Superresolution Imaging

    Conversion of plant biomass into soluble sugars is of high interest for production of fermentable industrial materials, such as biofuels. Biofuels are a very attractive alternative to fossil fuels, both for recycling of agricultural wastes and as a source of sustainable energy. Cellulosomes are among the most efficient enzymatic degraders of biomass known to date, due to the incorporation of a multiplicity of enzymes into a potent,...

    Lior Artzi, Tali Dadosh, Elad Milrot, Sarah Moraïs, Smadar Levin-Zaidman, Ely Morag, Edward A. Bayer
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