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ELISA

  • Open Access
    Decline of Humoral Responses against SARS-CoV-2 Spike in Convalescent Individuals
    Editor's Pick Observation | Therapeutics and Prevention
    Decline of Humoral Responses against SARS-CoV-2 Spike in Convalescent Individuals

    While waiting for an efficient vaccine to protect against SARS-CoV-2 infection, alternative approaches to treat or prevent acute COVID-19 are urgently needed. Transfusion of convalescent plasma to treat COVID-19 patients is currently being explored; neutralizing activity in convalescent plasma is thought to play a central role in the efficacy of this treatment. Here, we observed that plasma neutralization activity decreased a few weeks...

    Guillaume Beaudoin-Bussières, Annemarie Laumaea, Sai Priya Anand, Jérémie Prévost, Romain Gasser, Guillaume Goyette, Halima Medjahed, Josée Perreault, Tony Tremblay, Antoine Lewin, Laurie Gokool, Chantal Morrisseau, Philippe Bégin, Cécile Tremblay, Valérie Martel-Laferrière, Daniel E. Kaufmann, Jonathan Richard, Renée Bazin, Andrés Finzi
  • Open Access
    Dynamics of <span class="named-content genus-species" id="named-content-1">Mycobacterium tuberculosis</span> Ag85B Revealed by a Sensitive Enzyme-Linked Immunosorbent Assay
    Research Article | Host-Microbe Biology
    Dynamics of Mycobacterium tuberculosis Ag85B Revealed by a Sensitive Enzyme-Linked Immunosorbent Assay

    Bacterial protein secretion contributes to host-pathogen interactions, yet the process and consequences of bacterial protein secretion during infection are poorly understood. We developed a sensitive ELISA to quantitate a protein (termed Ag85B) secreted by M. tuberculosis and used it to find that Ag85B secretion occurs with slower kinetics than for proteins secreted...

    Joel D. Ernst, Amber Cornelius, Miriam Bolz
  • Open Access
    Research Article
    Nontypeable Haemophilus influenzae Lipooligosaccharide Expresses a Terminal Ketodeoxyoctanoate In Vivo, Which Can Be Used as a Target for Bactericidal Antibody
    Michael A. Apicella, Jeremy Coffin, Margaret Ketterer, Deborah M. B. Post, Christopher J. Day, Freda E.-C. Jen, Michael P. Jennings
  • Open Access
    Editor's Pick Research Article
    Influenza Virus Hemagglutinin Stalk-Specific Antibodies in Human Serum are a Surrogate Marker for In Vivo Protection in a Serum Transfer Mouse Challenge Model

    Influenza viruses are a serious concern for public health and cause a large number of deaths worldwide every year. Current influenza virus vaccines can confer protection from disease, but they often show low efficacy due to the ever-changing nature of the viruses. Novel vaccination approaches target conserved epitopes of the virus, including the hemagglutinin stalk domain, to elicit universally protective antibodies that also bind to...

    Henning Jacobsen, Madhusudan Rajendran, Angela Choi, Haakon Sjursen, Karl A. Brokstad, Rebecca J. Cox, Peter Palese, Florian Krammer, Raffael Nachbagauer
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