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CRISPR/Cas9

  • Open Access
    A Conditional Protein Degradation System To Study Essential Gene Function in <span class="named-content genus-species" id="named-content-1">Cryptosporidium parvum</span>
    Research Article | Host-Microbe Biology
    A Conditional Protein Degradation System To Study Essential Gene Function in Cryptosporidium parvum

    Cryptosporidium parvum and Cryptosporidium hominis are leading pathogens responsible for diarrheal disease (cryptosporidiosis) and deaths in infants and children below 5 years of age. There are no effective treatment options and no vaccine for cryptosporidiosis. Therefore, there is an urgent need to...

    Hadi H. Choudhary, Maria G. Nava, Brina E. Gartlan, Savannah Rose, Sumiti Vinayak
  • Open Access
    CRISPR/Cas9 Mutagenesis in <span class="named-content genus-species" id="named-content-1">Phlebotomus papatasi</span>: the Immune Deficiency Pathway Impacts Vector Competence for <span class="named-content genus-species" id="named-content-2">Leishmania major</span>
    Research Article | Host-Microbe Biology
    CRISPR/Cas9 Mutagenesis in Phlebotomus papatasi: the Immune Deficiency Pathway Impacts Vector Competence for Leishmania major

    Sand flies are the natural vectors of Leishmania parasites. Studies of sand fly/Leishmania interactions have been limited by the lack of successful genomic manipulation of these insects. This paper shows the first example of successful targeted mutagenesis in sand flies via adaptation of the CRISPR/Cas9 editing technique. We generated transmissible null mutant alleles of relish, a gene known to be...

    Isabelle Louradour, Kashinath Ghosh, Ehud Inbar, David L. Sacks
  • Open Access
    Optical DNA Mapping Combined with Cas9-Targeted Resistance Gene Identification for Rapid Tracking of Resistance Plasmids in a Neonatal Intensive Care Unit Outbreak
    Research Article | Clinical Science and Epidemiology
    Optical DNA Mapping Combined with Cas9-Targeted Resistance Gene Identification for Rapid Tracking of Resistance Plasmids in a Neonatal Intensive Care Unit Outbreak

    This study presents how a novel method, based on visualizing single plasmids using sequence-specific fluorescent labeling, could be used to analyze the genetic dynamics of an outbreak of resistant bacteria in a neonatal intensive care unit at a Swedish hospital. Plasmids are a central reason for the rapid global spread of bacterial resistance to antibiotics. In a single experimental procedure, this method replaces many traditional...

    Santosh K. Bikkarolla, Viveka Nordberg, Fredrika Rajer, Vilhelm Müller, Muhammad Humaun Kabir, Sriram KK, Albertas Dvirnas, Tobias Ambjörnsson, Christian G. Giske, Lars Navér, Linus Sandegren, Fredrik Westerlund
  • Open Access
    Research Article
    CRISPR/Cas9 Genome Editing Reveals That the Intron Is Not Essential for var2csa Gene Activation or Silencing in Plasmodium falciparum
    Jessica M. Bryant, Clément Regnault, Christine Scheidig-Benatar, Sebastian Baumgarten, Julien Guizetti, Artur Scherf
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