biochemistry
Research Article | Molecular Biology and PhysiologyMassively Parallel Fitness Profiling Reveals Multiple Novel Enzymes in Pseudomonas putida Lysine MetabolismP. putida lysine metabolism can produce multiple commodity chemicals, conferring great biotechnological value. Despite much research, the connection of lysine catabolism to central metabolism in P. putida remained undefined. Here, we used random barcode transposon sequencing to fill the gaps of...
- Research ArticleStructural Analysis of the Interaction between the Bacterial Cell Division Proteins FtsQ and FtsB
In most bacteria and archaea, filaments of FtsZ protein organize cell division. FtsZ forms a ring structure at the division site and starts the recruitment of 10 to 20 downstream proteins that together form a multiprotein complex termed the divisome. The divisome is thought to facilitate many of the steps required to make two cells out of one. FtsQ and FtsB are part of the divisome, with FtsQ being a central hub, interacting with most...
- Research ArticleThe NOX Family of Proteins Is Also Present in Bacteria
- Research ArticleRNA-Dependent Cysteine Biosynthesis in Bacteria and Archaea
Comprehensive analyses of all genomic and metagenomic protein sequence data in public databases revealed the distribution and evolution of an alternative cysteine-encoding system in diverse archaea and bacteria. The finding that the SepRS-SepCysS-SepCysE- and the selenocysteine-encoding systems are shared by the Euryarchaeota class I methanogens, the Crenarchaeota AK8/W8A-19 group, and an Asgard archaeon suggests that...