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biochemistry

  • Open Access
    Massively Parallel Fitness Profiling Reveals Multiple Novel Enzymes in <em>Pseudomonas putida</em> Lysine Metabolism
    Research Article | Molecular Biology and Physiology
    Massively Parallel Fitness Profiling Reveals Multiple Novel Enzymes in Pseudomonas putida Lysine Metabolism

    P. putida lysine metabolism can produce multiple commodity chemicals, conferring great biotechnological value. Despite much research, the connection of lysine catabolism to central metabolism in P. putida remained undefined. Here, we used random barcode transposon sequencing to fill the gaps of...

    Mitchell G. Thompson, Jacquelyn M. Blake-Hedges, Pablo Cruz-Morales, Jesus F. Barajas, Samuel C. Curran, Christopher B. Eiben, Nicholas C. Harris, Veronica T. Benites, Jennifer W. Gin, William A. Sharpless, Frederick F. Twigg, Will Skyrud, Rohith N. Krishna, Jose Henrique Pereira, Edward E. K. Baidoo, Christopher J. Petzold, Paul D. Adams, Adam P. Arkin, Adam M. Deutschbauer, Jay D. Keasling
  • Open Access
    Research Article
    Structural Analysis of the Interaction between the Bacterial Cell Division Proteins FtsQ and FtsB

    In most bacteria and archaea, filaments of FtsZ protein organize cell division. FtsZ forms a ring structure at the division site and starts the recruitment of 10 to 20 downstream proteins that together form a multiprotein complex termed the divisome. The divisome is thought to facilitate many of the steps required to make two cells out of one. FtsQ and FtsB are part of the divisome, with FtsQ being a central hub, interacting with most...

    Danguole Kureisaite-Ciziene, Aravindan Varadajan, Stephen H. McLaughlin, Marjolein Glas, Alejandro Montón Silva, Rosa Luirink, Carolin Mueller, Tanneke den Blaauwen, Tom N. Grossmann, Joen Luirink, Jan Löwe
  • Open Access
    Research Article
    cAMP Receptor Protein Controls Vibrio cholerae Gene Expression in Response to Host Colonization
    Jainaba Manneh-Roussel, James R. J. Haycocks, Andrés Magán, Nicolas Perez-Soto, Kerstin Voelz, Andrew Camilli, Anne-Marie Krachler, David C. Grainger
  • Open Access
    Research Article
    The NOX Family of Proteins Is Also Present in Bacteria
    Christine Hajjar, Mickaël V. Cherrier, Gaëtan Dias Mirandela, Isabelle Petit-Hartlein, Marie José Stasia, Juan C. Fontecilla-Camps, Franck Fieschi, Jérôme Dupuy
  • Open Access
    Research Article
    RNA-Dependent Cysteine Biosynthesis in Bacteria and Archaea

    Comprehensive analyses of all genomic and metagenomic protein sequence data in public databases revealed the distribution and evolution of an alternative cysteine-encoding system in diverse archaea and bacteria. The finding that the SepRS-SepCysS-SepCysE- and the selenocysteine-encoding systems are shared by the Euryarchaeota class I methanogens, the Crenarchaeota AK8/W8A-19 group, and an Asgard archaeon suggests that...

    Takahito Mukai, Ana Crnković, Takuya Umehara, Natalia N. Ivanova, Nikos C. Kyrpides, Dieter Söll
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