alternative splicing
Research Article | Molecular Biology and PhysiologyCombination of Proteogenomics with Peptide De Novo Sequencing Identifies New Genes and Hidden Posttranscriptional ModificationsNext-generation sequencing techniques have considerably increased the number of completely sequenced eukaryotic genomes. These genomes are mostly automatically annotated, and ab initio gene prediction is commonly combined with homology-based search approaches and often supported by transcriptomic data. The latter in particular improve the prediction of intron splice sites and untranslated regions. However, correct prediction of...