TABLE 1 

HfsK and CC1244 detection by CCMS screening for c-di-GMP effectors

Protein and exptaNo. of spectral counts of identified peptides (CCMS experiment/CCMS competition)b
Soluble fractionMembrane fraction
HfsK (CC3689)
    19/014/5
    28/013/4
    310/014/4
CC1244
    14/01/0
    20/02/1
    30/08/3
  • a Results of three independent experiments using 10 μM (soluble fraction) or 8 μM (membrane fraction) c-di-GMP capture compound are shown.

  • b Competition experiments were performed in the presence of 1 mM c-di-GMP.