TABLE 1 

Influence of selected transposon mutants of P. aeruginosa PA14 on abscess infections

StrainProduct or functionArea of dermonecrosis (mean ± SE; mm2)Geometric mean CFU per abscessMouse survival rate (%)Infected kidney (%)Infected liver (%)
P. aeruginosa PA14Wild type76 ± 151.23 × 108608050
P. aeruginosa fliI Flagella/motility23 ± 13a3.03 × 1071000b0
P. aeruginosa wzz LPS O-antigene121 ± 921.49 × 108757575
P. aeruginosa fleQ Matrix PS regulationf122 ± 702.13 × 1087550100
P. aeruginosa rhlR Rhl quorum sensing131 ± 411.42 × 107a1005025
P. aeruginosa lasI Las quorum sensing129 ± 313.34 × 1081007550
P. aeruginosa ΔpchAD/ΔpvdGLcPyochelin/pyoverdine0a1.63 × 103a1000b0
P. aeruginosa lipA Lipase141 ± 1174.01 × 10850100100
P. aeruginosa lasB Elastase47 ± 233.29 × 107507575
P. aeruginosa toxA Exotoxin A48 ± 432.47 × 108505050
P. aeruginosa exsA Type III secretion regulation1 ± 1a1.78 × 105a1000b0
P. aeruginosa exoU Exoenzyme U34 ± 171.31 × 1071005050
P. aeruginosa phzA2 Phenazine75 ± 191.46 × 108757575
P. aeruginosa algR Global regulator95 ± 221.47 × 108100250
P. aeruginosa bfiS Biofilm 2-component regulatorn.d.d1.22 × 109d0100100
P. aeruginosa phoP 2-component response regulator of PhoPQ53 ± 62.66 × 108755050
P. aeruginosa phoQ 2-component sensor kinase138 ± 522.07 × 108605050
  • a Significant difference from the wild type (P < 0.05; two-tailed Mann-Whitney test).

  • b significant difference from the wild type (P < 0.05; two-tailed Fisher exact test).

  • c PA14 pyochelin/pyoverdine deletion mutant.

  • d Mice with very low survival. The data reflect only surviving animals, and no statistics could be applied. n.d., not determined.

  • e LPS, lipopolysaccharide.

  • f PS, polysaccharide.