TABLE 1 

List of ppGalNAc-T2 substrate candidate proteins identified from the jacalin affinity purificationa

Identified proteinAccession no.Predicted O-GalNAcPeptide count
WTΔt2WT/Δt2
CST1TGME49_26466019796713.71
HypotheticalTGME49_3132706742172.55
HypotheticalTGME49_230940265393.511.14
MIF4G domain containingTGME49_269180153317.54.07
HypotheticalTGME49_2281201172554.90
GYF domain containingTGME49_20416037145.52.55
VSP13TGME49_291180239145.52.55
GYF domain containingTGME49_29861098120NA
GRA7TGME49_20331019.533.17
Kelch repeat containingTGME49_229000119.524.75
Sec7 domain containingTGME49_2321901399.533.17
GRA3TGME49_22728048.532.83
HypotheticalTGME49_31337074824.00
HypotheticalTGME49_2159101427.50NA
HypotheticalTGME49_2884605370NA
HypotheticalTGME49_2874802516.50NA
Perforin-like protein PLP1TGME49_20413026616.00
LsmAD domain containingTGME49_2314406660NA
Pyrroline-5-carboxylate reductaseTGME49_2360709623.00
Thioredoxin reductaseTGME49_30973022623.00
HypotheticalTGME49_3112107160NA
Lysine decarboxylase familyTGME49_23657075.50NA
  • a The list is sorted by the number of peptides detected in wild type (WT). Predicted O-GalNAc, number of glycosylation sites predicted by NetOGlyc 4.0; WT and ppGalNAc-Δt2, normalized peptide count detected in mass spectrometry; WT/Δt2, ratio of normalized peptide count between wild type and Δt2 sample (NA represents no count detected in Δt2 sample). The list was filtered with the semiquantitative criteria (proteins that have more than 5 peptides mapped in wild type with more than a 2.5-fold-increased detection in wild type over Δt2 sample).