TABLE 2 

List of ppGalNAc-T2 substrate candidate proteins identified from the VVA affinity purificationa

Identified proteinAccession no.Predicted O-GalNAcPeptide count
WTΔt2WT/Δt2
IMC2ATGME49_2281706440NA
VSP13TGME49_291180239240NA
Sec7 domain containingTGME49_232190139180NA
HypotheticalTGME49_212300101635.33
GRA7TGME49_2033101150NA
HypotheticalTGME49_223070490NA
Cyclophilin precursorTGME49_205700680NA
SRS34ATGME49_27105012832.67
CST1TGME49_26466019770NA
HypotheticalTGME49_2895402670NA
Sortilin, putativeTGME49_2901602270NA
MIC17ATGME49_200250760NA
HypotheticalTGME49_201180360NA
  • a The list is sorted by the number of peptides detected in wild type (WT). Predicted O-GalNAc, number of glycosylation sites predicted by NetOGlyc 4.0; WT and ppGalNAc-Δt2, normalized peptide count detected in mass spectrometry; WT/Δt2, ratio of normalized peptide count between wild type and Δt2 sample (NA represents no count detected in Δt2 sample). The list was filtered with the semiquantitative criteria (proteins that have more than 5 peptides mapped in wild type with more than a 2.5-fold-increased detection in wild type over Δt2 sample).