TABLE 2

Predicted outer membrane proteins and sequence similarity in N. gonorrhoeae and NmB strains

NEIS no.Protein nameaIdentified in OMVsAmino acid sequence similarity (%)Experimentally tested as GC vaccinec
Between N. gonorrhoeae and NZ98/254bWithin N. gonorrhoeae
RangeMean ± SDRangeMean ± SD
NEIS2109NhbA67.5–8872.8 ± 5.278.3–10096.8 ± 5.0
NEIS1183GNA103090.9–93.592.2 ± 0.998.4–10099.1 ± 0.5
NEIS2020PorB+66.2–94.269.7 ± 2.965.2–10091.3 ± 6.2+
NEIS1963FetA+90.5–9592.8 ± 1.489.5–10096.4 ± 1.9
NEIS0408PilQ+89.3–97.293.1 ± 2.395.7–10099.2 ± 0.6+
NEIS0173Omp85 (BamA)+95–95.895.2 ± 0.299–10099.6 ± 0.2+
NEIS1783RmpM+92.6–93.693.2 ± 0.398.7–10099.4 ± 0.3
NEIS2198OpcA+44.8–47.145.4 ± 0.697.7–10099 ± 0.5+
NEIS0612NspA+90.8–95.794 ± 0.992–10098.2 ± 1.5+
NEIS1632MtrE+93–97.594.9 ± 1.795–10099.1 ± 1.3+
NEIS0586MafB179–80.979.5 ± 0.496–10099.2 ± 1.1
NEIS1917MetQ+96.5–99.396.9 ± 0.797.2–10099.6 ± 0.9+
NEIS0653ComL+98.1–99.698.4 ± 0.497.8–10099.4 ± 0.6
NEIS1687OM phospholipase A precursor+98.4–98.998.6 ± 0.199.2–99.799.5 ± 0.2
NEIS1468LbpA+86.3–96.395.5 ± 1.887.9–10098.3 ± 2.4+
NEIS1487FkpA (macrophage infectivity protein)+96.7–99.297.3 ± 0.696–10098.9 ± 1.0
hmbRHmbR89.5–89.689.5 ± 0.1100–100100 ± 0.0
NEIS0566LolB98.1–98.598.4 ± 0.299–10099.7 ± 0.3
NEIS1418NlpD90.3–9493.1 ± 0.795.4–10099.2 ± 0.7
NEIS1462H.8 OMP+95.1–98.397.8 ± 0.888.6–10097.6 ± 3.8
NEIS1933VacJ-related protein94.9–95.695.3 ± 0.295.6–10098.4 ± 1.6
NEIS0338Ferric siderophore receptor protein74.9–97.292.9 ± 7.576.6–10094.3 ± 8.8
NEIS1813OstA93.2–94.393.5 ± 0.498.9–10099.2 ± 0.3
NEIS1691TbpB60.8–86.367.3 ± 5.358.9–10075.9 ± 7.7+
NEIS1549AniA83.4–92.384.4 ± 2.495.8–10099.2 ± 0.6+
NEIS1205Ape194.5–97.495.3 ± 0.695.2–10098.3 ± 1.3
NEIS2124Lipoprotein98.6–99.398.9 ± 0.398.6–10099.1 ± 0.4
NEIS1063Putative periplasmic protein+96.3–96.796.6 ± 0.299.1–10099.7 ± 0.3
NEIS1066Putative periplasmic protein+93.3–94.193.8 ± 0.398.7–10099.4 ± 0.3
NEIS0275Putative OM solvent tolerance protein (LPS assembly protein)+89.1–89.989.5 ± 0.292.6–10099.4 ± 1.1
NEIS0944Putative OM receptor protein (TonB-dependent receptor)+96.7–97.396.9 ± 0.199.4–10099.7 ± 0.1
NEIS1428Putative OM substrate binding protein (TonB-dependent receptor)+94.8–96.595.8 ± 0.497–10098.6 ± 0.6
NEIS0739Putative amino acid permease substrate-binding protein+97.1–98.597.8 ± 0.398.6–10099.4 ± 0.4
NEIS1920Putative transglycosylase+95.9–97.897.4 ± 0.396.3–10099.1 ± 0.6
NEIS2112Putative OMP98.4–99.198.9 ± 0.298.1–10099.5 ± 0.4
NEIS0261Putative periplasmic protein86.2–9793.5 ± 2.885.4–10097.1 ± 3.7
NEIS0504Putative thiamine biosynthesis protein96–96.396.2 ± 0.199.4–10099.8 ± 0.2
NEIS0630Putative periplasmic protein92.1–92.692.2 ± 0.299–10099.4 ± 0.3
NEIS0729Putative secreted protein91.4–96.393.9 ± 1.392.9–10098 ± 1.9
NEIS1304Putative membrane lipoprotein96.7–97.297.1 ± 0.299.5–10099.8 ± 0.3
NEIS1195Putative peptidyl-prolyl cis-trans isomerase A92.4–94.593.6 ± 0.697.3–10099.1 ± 0.7
NEIS1935Putative periplasmic transport protein91.8–92.892.2 ± 0.498.5–10099.1 ± 0.4
NEIS0196Putative OM lipoprotein+92.8–95.293.4 ± 0.991.2–99.295.8 ± 3.6
NEIS1084Putative periplasmic protein+93.1–9593.7 ± 0.697–10098.3 ± 0.8
NEIS1125Putative periplasmic protein95.3–9795.8 ± 0.698.8–10099.1 ± 0.3
NEIS1172Putative periplasmic protein92.7–99.793.2 ± 1.192.7–10099.1 ± 1.4
NEIS1367Putative lipoprotein96.1–96.696.2 ± 0.398.9–10099.2 ± 0.4
NEIS1271Hypothetical protein+95.5–97.196.4 ± 0.497.3–10099 ± 0.5
NEIS1858Hypothetical protein92.4–99.194.5 ± 1.591.6–10098.3 ± 1.9
NEIS1945Hypothetical protein74.2–99.282.3 ± 8.374.0–10093.5 ± 9.2
NEIS1485Hypothetical protein97.3–10097.7 ± 0.797.3–10099 ± 0.9
NEIS1546Hypothetical protein88.4–97.796.1 ± 2.287.0–10097.9 ± 3.4
NEIS1746Hypothetical protein62.7–87.767 ± 9.398.4–10099 ± 0.5
NEIS2446Hypothetical protein83.5–91.288.1 ± 2.490.1–10096.5 ± 2.7
NEIS0790Hypothetical protein74.6–80.676.7 ± 2.293–10097.7 ± 2.6
NEIS1136Hypothetical protein82.6–83.783.1 ± 0.597.8–10098.9 ± 0.6
NEIS1253Hypothetical protein91.9–97.593.4 ± 1.393.8–10098.1 ± 1.5
  • a Previously reported proteins from detergent-extracted NZ98/254 outer membrane vesicles (OMVs) (24, 30, 32, 34). Proteins in boldface are most abundantly expressed, and their expression was not significantly expressed across different OMV lots (32). OM, outer membrane; LPS, lipopolysaccharide; OMP, outer membrane protein.

  • b Values in boldface show mean sequence similarity that is greater than 80%.

  • c Elicits bactericidal antibodies to N. gonorrhoeae (9, 12, 13, 41). GC, gonorrhea.