TABLE 1

List of bacterial strains and plasmids used in this studya

Strain or plasmidRelevant genotype or phenotypeSource and/or reference
Strains
    E. coli ATCC 15144Wild-type strainLeibniz Institute DSMZ—German Collection of
Microorganisms and Cell Cultures,
Braunschweig, Germany
    E. coli ECOR1-72The E. coli reference collection of natural isolatesSTEC Center, Michigan State University, USA
    E. coli BL21(DE3)F ompT gal dcm lon hsdSB(rB mB) λ(DE3 [lacI lacUV5-T7p07
ind1 sam7 nin5]) [malB+]K-12S)
Laboratory collection
    E. coli ATCC 11303Wild-type strain for T1 phage propagationLeibniz Institute DSMZ—German Collection of
Microorganisms and Cell Cultures
(Braunschweig, Germany)
    E. coli MG1655F lambda ilvG rfb-50 rph-1Laboratory collection
    E. coli YC55MG1655 ΔcyaA KmsThis work
    E. coli YC170MG1655 ΔcyaA Δcrp KmrThis work
    E. coli W3110W3110 derivative with functional RpoS [rpoS396(Am)]54
    E. coli VS823W3110 ΔluxS Kms29
Plasmids
    pTrc99AAmpr; expression vector; pBR ori; trc promoter, IPTG inducible55
    pUA66Kmr; expression vector; SC101 ori, GFPmut2 under the control
of promoter of interest
56
    pLeoL9Ampr; cor in pTrc99A, IPTG inducibleThis work
    pLeoL10Ampr; pir in pTrc99A, IPTG inducibleThis work
    pLeoL11Ampr; orf65 in pTrc99A, IPTG inducibleThis work
    pLeoL12Kmr; Phol-gfp in pUA66This work
    pLeoL13Kmr; Pdam-gfp in pUA66This work
    pLeoL14Kmr; Ppir-gfp in pUA66This work
    pLeoL15Kmr; PrecE-gfp in pUA66This work
    pLeoL16Kmr; Porf65-gfp in pUA66This work
    pVS1723Kmr; Plsr-gfp in pUA6629
    pYC75Camr; crp with C-terminal His6 tag under the control of IPTG-
inducible T5-lac promoter in pCA24N expression vector
This work and reference 57
    pAL60Camr, gfp under the control of a synthetic constitutive promoter
J23101 in pSB1C3 expression vector
This work and Anderson Promoter Collection
    pdCas9aTc-inducible expression of a catalytically inactive bacterial Cas9
(Streptococcus pyogenes) for bacterial gene knockdown
Addgene, USA; catalogue no. 44249
    pgRNAExpression of customizable guide RNA (gRNA) for bacterial gene
knockdown
Addgene, USA; catalogue no. 44251
  • a pgRNA, pregenomic RNA; Kms, kanamycin sensitive; Kmr, kanamycin resistant; Ampr, ampicillin resistant; Camr, chloramphenicol resistant.