TABLE 1

Primers and probe used in this study

NameOrientationSequence
GalK-Kan insertion
    US28-2x Stop and US28-
Y16F
FwdCGTGGACCAGGCGGTGTCCATGCACCGAGGGCAGAACTGGTGCTACC
ATGCCTGTTGACAATTAATCATCGGCATAG
RevGTCGTATTCAAACTCCGTCGTGAGTTCCGTG
GTCGTCGTCGTCGGCGTCGGCGTCTCAGCAAAAGTTCGATTTA
    US28-R129AFwdCCGTGTACGTTACTCACTGCCTGTTTCTACGTGGCTATGTTTGCCAGT
TTGTGTTTTATTACGGAGATTGCACTCCCTGTTGACAATTAATCATCG
GCATAG
RevGGCAAAGATCCACCAAAAAATACTGAAAAGGCAGGCCTGTTTTACAG
GCCGATATCTCATGTAAACAATAGCGTACTCAGCAAAAGTTCGATTTA
    US28-ddFKBPFwdACAGCATGAGCTTTTCGCGTCGGAGCTCGCCGAGCCGAAGAGAGAC
GTCTTCCGACACGCTGTCCGACGAGGTGTGTCGCGTCTCACAAATTA
TACCGCCTGTTGACAATTAATCATCGGCATAG
RevTAAAAAAGCGCTACCTCGGCCTTTTCATACAAACCCCGTGTCCGCCC
CTTTTTTCCCCGTGCCCGATATACACGATATTAAACCCACGACCATTT
CCGTTCGATTCTCAGCAAAAGTTCGATTTA
Replacement
    US28-2x StopFwdCTCTTTCACGCGTCCGCCGCACA
RevGAGCATTGAATCCGACGTCGC
    US28-Y16FFwdGGTGAACCGCTCATATAGACCA
RevCAAGAAGTTGCCGACGGAAC
    US28-R129AFwdACAACTCCCTAGCCAGCGTGCCGTGTACGTTACTCACTGCC
    RevTTGGTCACCACCATAAAGTGTGGAATGGCGATGATCAC
    US28-ddFKBPFwdTCGCCGAGCCGAAGAGAGACATCTTCCGACACGCTGTCCGACGAGG
TGTGTCGCGTCTCACAAATTATACCGATGGGAGTGCAGGTGGAAACCA
RevGGGCGGACACGGGGTTTGTATGAAAAGGCCGAGGTAGCGCTTTTTTA
TTACTCAGCAAAAGTTCGATTTAGAATTCTTCCGGTTTTAGAAGCTCCA
HCMV detection
    UL141FwdGATGTGGGCCGAGAATTATGA
RevATGGGCCAGGAGTGTGTCA
ProbeFAM-CGAGGGAGAGCAAGTT-MGB