TABLE 1

Strains and plasmids used in this study

Strain or plasmidRelevant genotype and/or characteristic(s)Reference or source
P. aeruginosa strains used
in the infectiona
    PA14-1Barcode CAAAAGGACA; Gent
    PAO1-B11Barcode GTGTCGTGGG; Gent
    B23-2Wound isolate; barcode GCCTATTGTG; GentLubbock, TX
    CF18-1Nonmucoid CF isolate; barcode GTTACGTCAA; Gent60
    MSH10-2Water isolate; barcode TATCAGATTT; Gent60
    S54485-1UTIb isolate; barcode TTAAACTAGG; Gent60
P. aeruginosa strains
    PA14
    PA14ΔwspFClean wspF deletion
    PAO1
    PAO1ΔwspFClean wspF deletion (JJH356)
    PA14-1attB::lacZlacZ from miniCTX-lacZ introduced at the attB siteThis study
    RSCV-2 wt-wspAVariant wspA replaced by the wild-type alleleThis study
    RSCV-1 wt-wspAVariant wspA replaced by the wild-type alleleThis study
    RSCV-40 wt-wspAVariant wspA replaced by the wild-type alleleThis study
E. coli strains
    NEB5-αNEBc
    S17NEB
Plasmids
    pEX18ApGene deletion vector47
    pEX18Ap::wt-wspAwspA-complementing constructThis study
    pCdrA::gfpCdrA promoter fused to gfp; Carb31
    pMH487Empty vector for pCdrA::gfp; Carb31
    pJN2133PA2133 cloned into pJN105; Gent32
    pHERD20TEmpty vector; Carb56
    pHERD2133PA2133 from pJN2133 cloned into pHEDR20T; CarbThis study
    miniCTX-lacZTet58
  • a All strains used in the infection are resistant to gentamicin. Barcodes are located at the Tn7 site on the genome.

  • b UTI, urinary tract infection.

  • c NEB, New England Biolabs.