TABLE 1

Elements of the ferroverdin/bagremycin BGC in S. lunaelactis and comparative analysis of similar clusters in other Streptomyces speciesa

Gene ID
(SLUN)
NamebPredicted
function
fev ID in
Streptomyces sp.
WK-5344
bag ID in
Streptomyces sp.
Tü-4128; accession no.
gri in
S. griseus
subsp. griseus
nsp in
S. murayamaensis
21345AminotransferaseNA23415 (NS);
WP_122618577
NFNF
21350ORF1Prephenate dehydrogenase89–93;
BAM73624
Pseudo-23410NFNF
21355fevLDecarboxylase88–93;
BAM73625
88–93 (bagN); 23405/
WP_122618385
NFNF
21360fevKDecarboxylase82–89;
BAM73626
82–89 (bagM); 23400/
WP_122618384
NFNF
21365fevMTranscriptional regulator,
MarR family
90–92;
BAM73627
90–92 (bagL); 23395/
WP_122618383
NFNF
21370fevA1FAD-dependent
oxygenase
79–86;
BAM73628
78–86 (bagK); 23390/
WP_122618382
57–69 (griA);
BAF36643
51–65 (nspA);
BAJ08166
21375fevBPutative bagremycin
transporter
88–93;
BAM73629
88–93 (bagJ); 23385/
WP_122618381
57–71 (griB);
BAF36644
NS (orf2);
BAJ08168
21380fevRSARP-family
transcriptional
activator
81–87;
BAM73630
80–86 (bagI); 23380/
AKA27633.1
47–64 (griR);
BAF36642
53–68 (nspR);
BAJ08169
21385fevFo-Aminophenol
oxidase
89–93;
BAM73631
89–93 (bagH); 23375/
WP_122618379
68–78 (griF);
BAF36648
60–74 (nspF);
BAJ08174
21390fevECopper chaperon73–79;
BAM73632
Pseudo-23370 (bagZ)61–74 (griE);
BAF36647
57–68 (nspE);
BAJ08173
21395fevA2FAD-dependent
oxygenase
83–90;
BAM73633
83–90 (bagG); 23365/
WP_122618378
56–69 (griA);
BAF36643
52–65 (nspA);
BAJ08166
21400fevYPhospho-2-dehydro-3-
deoxyheptonate
aldolase
88–94;
BAM73634
88–94 (bagF); 23360/
WP_122618377
NFNF
21405fevWBagremycin synthetase84–89;
BAM73635
84–89 (bagE); 23355/
WP_122618576
NFNF
21410fevVTyrosine ammonia-lyase92–95;
BAM73636
91–95 (bagA); 23350/
WP_122618575
NFNF
21415fevIDhnA-type aldolase91–96;
BAM73637
91–96 (bagB); 23345/
WP_122618376
72–85 (griI);
BAF36651
74–85 (nspI);
BAJ08171
21420fevH3,4-AHBA synthase92–96;
BAM73638
92–96 (bagC); 23340/
WP_122618375
77–87 (griH);
BAF36650
79–89 (nspH);
BAJ08172
21425fevOHypothetical protein86–95;
BAM73639
85–94; 23335/
WP_122618374
NFNF
21430fevTLuxR-family transcriptional
regulator
84–92;
BAM73640
84–92 (bagY); 23330/
WP_122618373
63–78 (griT);
BAF36640
62–76 (nspT);
BAJ08165
21435ORF2Carboxymuconolactone
decarboxylase
NS; BAM73641Pseudo-23325NFNF
  • a bag, bagremycin cluster (NZ_QTSY01000069) (DXM28_RS); fev, ferroverdin cluster (AB689797); gri, grixazone cluster (AB259663); nsp, nitrosobenzamide cluster (AB530136). Abbreviations: ID, identifier; sim, similarity; NA, sequence not available; NS, not similar; NF, none found.

  • b Gene names were provided based on the fev cluster of Streptomyces WK-5344 (GenBank accession no. AB689797.1). Paired numbers refer to the percentage of amino acid identity and similarity, respectively.