TABLE 2

Proviral mutation frequency in BALB/c T-cell lymphomas induced by TBLV-WT and TBLV-SD

Mutation
type
Mutation frequencyaFold increase
(SD/WT)
TBLV-WTTBLV-SD
G to A2.022.981.5
A to G0.601.272.1
C to T0.080.739.1
T to C0.861.531.8
Transitions3.566.511.8
Transversions0.100.717.1
WRC0.260.672.6
SYC0.140.694.9
TYC0.841.652.0
ATC0.180.472.6
  • a Number of mutations/number of clones was determined on the basis of Sanger sequencing of 1,100 bp of the plus strand of at least fifteen proviral env gene clones obtained from each of three independent BALB/c tumors induced by TBLV-WT (n = 50) or TBLV-SD (n = 55). Frequencies of WRC, SYC, TYC, and ATC-motif mutations were calculated from both strands. W = A or T; R = A or G; S = G or C; Y = C or T. For statistical analysis of the data, see scatter plots in Fig. 3. Boldface data highlight the greatest differences between MMTV-WT and MMTV-SD sequences typical of AID-mediated mutagenesis.