TABLE 3

Experimental data

StrainDescriptionNo. of
BPSs
No. of MA
lines
Total no. of
generations
BPSs per
generation
× 103
95%
CLd
CollectiveaMMR30,061334264,9581132
PFM421ΔrnhA ΔmutL3,1983922,6901417
PFM669ΔmutL (AB1157)1,3343318,850716
PFM430/431oriC+ oriZ+ ΔmutL (AB1157)3,1506533,607946
PFM426ΔoriC oriZ+ ΔmutL (AB1157)3,5444525,1761419
PFM533/534ΔseqA ΔmutL1,8554420,927897
PFM5maΔmutL on minimal medium1,4354828,197513
PFM343maΔmutS on minimal medium1,6084626,280614
PFM343VBsaΔmutS on supplemented minimal medium2,4763923,0171085
PFM343dLBaΔmutS on diluted LB3,5464022,4951588
PFM342LTMaΔmutS at low temperature1,2594424,875515
PFM799ΔnrdR ΔmutL5,2724223,58122412
PFM677Δrep ΔmutL3,2584323,9611367
PFM256Δtus ΔmutL3,6722832,96711110
PFM257ΔmatP ΔmutL3,9793336,7251087
PFM422ΔrecA ΔmutL6,7974039,71317111
PFM424ΔrecA ΔmutS6,2663636,34017211
PFM456ΔrecB ΔmutL3,3214620,5981619
PFM118aΔumuDC ΔdinB ΔmutL1,1102312,0789241
PFM120lexA3 ΔsulA ΔmutL2,9093319,85314713
PFM259ΔhupB ΔmutL3,9103236,3901078
PFM258ΔhupA ΔmutL2,0752627,315767
PFM317/318Δfis ΔmutL7,6163537,73120214
PFM482Δfis ΔmutS4,6694022,68720612
PFM741Δhns ΔmutL3,3674625,5511325
PFM713Δdps ΔmutL2,9274023,3771258
PFM163bmutD513,625263,4813,915344
PFM165/397/399bmutD5 ΔmutL40,686757,0125,802456
CollectiveaWild Type1,9333412,015,0660.960.10
CollectivecBacillus subtilis mutS::Tn1010,55171171,805613
  • a Data are from reference 11.

  • b Data are from reference 10.

  • c Data represent combined results from four MA experiments performed with DK2140 (NCIB3610 mutS::Tn10), DK2141 (NCIB3610 mutS::Tn10), DK2142 (NCIB3610 mutS::Tn10 ΔcomI), and DK2143 (NCIB3610 mutS::Tn10 ΔcomI). There were no differences in mutation rates or spectra among these strains.

  • d 95% CL, 95% confidence limits to the BPSs per generation.