TABLE 2

Effect of gene loss on respiration rate

ClassStrainGenotypeRespiration
ratea
% inhibition
KCNbSHAMc
Wild typeSN152Wild type145.7 ± 6.495.6 ± 1.91.9 ± 0.8
IGoa1ms-25goa1Δ/Δ51.7 ± 4.760.4 ± 2.629.4 ± 2.7
mt1179-21nuo3Δ/Δ54.4 ± 5.661.9 ± 2.927.3 ± 3.4
mt5077-21nuo4Δ/Δ55.1 ± 7.267.5 ± 3.222.9 ± 3.8
mt2819-21nue1Δ/Δ52.7 ± 2.562.7 ± 4.026.4 ± 7.6
mt4467-25nue2Δ/Δ51.3 ± 9.366.2 ± 5.418.8 ± 3.6
mt2513-21mne1Δ/Δ49.8 ± 3.167.2 ± 3.424.2 ± 3.1
IImt6918-24qce1Δ/Δ12.1 ± 0.714.6 ± 4.467.9 ± 4.5
mt1371-21coe1Δ/Δ11.8 ± 0.814.9 ± 4.965.2 ± 4.7
mt6566-21coe2Δ/Δ10.9 ± 1.614.6 ± 5.467.8 ± 5.8
mt230-21pet111Δ/Δ10.8 ± 1.516.2 ± 1.764.2 ± 3.7
IIImt102-22aep1Δ/Δ8.2 ± 1.239.2 ± 3.614.9 ± 6.8
  • a Units represent nanomoles of O2/minute/milliliter·OD595 ± standard errors of the means (SEM) from at least 3 independent determinations.

  • b Data represent levels of inhibition of respiration by 1 mM potassium cyanide.

  • c Data represent levels of inhibition of respiration by 5 mM salicylhydroxamic acid.