TABLE 2

Strains and plasmid used in this study

Strain or plasmidDescriptionReference or source
K. kingae strains
    KK01Nonspreading/noncorroding derivative of 269-4925
    KK01 csaAKK01 with csaA deletion12
    KK01 pamKK01 with pamABCDE deletion11
    KK01 csaA pamKK01 with csaA deletion and pamABCDE deletion10
    KK01 rtxKK01 with rtxBDCA deletionThis study
    KK01 csaA rtxKK01 with csaA deletion and rtxBDCA deletionThis study
    KK01 pam rtxKK01 with pam deletion and rtxBDCA deletionThis study
    KK01 csaA pam rtxKK01 with csaA deletion, pam deletion, and rtxBDCA deletionThis study
E. coli strain
    DH5αλ ϕ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK mK) supE44 thi-1 gyrA relA147
Plasmid
    pUC19rtxBDCA::kanpUC19 with aphA3 kanamycin cassette flanked by surrounding 5′ and 3′ regions of the rtxBDCA locusThis study