TABLE 1

A. fumigatus strains used in this study

DescriptionTarget geneRelevant genotypeaReference
or source
Wild typeCEA1062
ΔakuB (recipient strain)AFUB_019720CEA17 akuB::pyrG; PyrG+63
ΔmpkAAFUB_070630ΔakuB mpkA::ptrA; PTR36
mpkAc (complemented ΔmpkA strain)ΔakuB ΔmpkA ΔmpkA::mpkA-hph; HygR PTR36
ΔmpkBAFUB_078810ΔakuB mpkB::ptrA; PTRThis study
ΔmpkB::mpkB+ΔakuB ΔmpkB ΔmpkB::mpkB-hph; HygRThis study
ΔpksPAFUB_033290ΔakuB pksP::hph; HygR35
ΔmpkB ΔpksPΔakuB ΔmpkB pksP::hph; HygR PTRThis study
ΔmpkA ΔmpkBΔakuB ΔmpkB mpkA::ptrA; HygR PTRThis study
ΔgpaAAFUB_012620ΔakuB gpaA::ptrA; PTRThis study
gpaAcΔakuB ΔgpaA gpaA-hph; HygR PTRThis study
ΔgpaA ΔpksPΔakuB ΔgpaA pksP::hph; HygR PTRThis study
ΔgpaBAFUB_012410ATCC 46645 gpaB::hph; HygR39
ΔgpaCAFUB_036760ΔakuB gpaC::ptrA; PTRThis study
ΔgprMAFUB_090880ΔakuB gprM::ptrA; PTRThis study
gprMcΔakuB ΔgprM ΔgprM::gprM-hph; HygRThis study
ΔgprM ΔpksPΔakuB ΔgprM pksP::hph; HygR PTRThis study
mpkB-gfpΔakuB mpkB::mpkB-gfp-hph; HygRThis study
ΔmpkA mpkB-gfpΔakuB mpkB-gfp mpkA::ptrA; HygR PTRThis study
gpaA-gfp gprM-3×HACEA10 gpaA-gfp gprM-3×HA; PTRThis study
gfp gprM-3×HACEA10 gfp gprM-3×HA; PTRThis study
ΔhmgAAFUB_021290ΔakuB hmgA::ptrA; PTR32
ΔhppDAFUB_021270ΔakuB hppD::ptrA; PTR32
ΔgprAAFUB_034900ΔakuB gprA::hph; HygRThis study
ΔgprBAFUB_055410ΔakuB gprB::hph; HygRThis study
ΔgprCAFUB_090350ΔakuB gprC::hph; HygRThis study
ΔgprDAFUB_028290ΔakuB gprD::hph; HygRThis study
ΔgprHAFUB_052640ΔakuB gprH::hph; HygRThis study
ΔgprIAFUB_047630ΔakuB gprI::hph; HygRThis study
ΔgprJAFUB_007220ΔakuB gprJ::hph; HygRThis study
ΔgprKAFUB_101830ΔakuB gprK::hph; HygRThis study
ΔgprOAFUB_038590ΔakuB gprO::hph; HygRThis study
ΔgprPAFUB_073100ΔakuB gprP::hph; HygRThis study
ΔnopAAFUB_088000ΔakuB nopA::hph; HygRThis study
  • a HygR, hygromycin; PTR, pyrithiamine.