TABLE 1

Expression and purification of G. diazotrophicus dioxygenase

StepVol (ml)Protein
(mg/ml)
Total protein
(mg)
Activitya
(µM · min−1)
Sp act (µmol ·
min−1 · mg−1)
Purification
fold
Yield (%)
Crude extractb6.015.995.41.5780.5951.0100
Ni2+ chromatography7.04.531.80.8231.0871.861
  • a Activity was determined using a 50-µl protein solution under the assay conditions.

  • b Crude extract was obtained from a cellular paste harvested from a 0.5-liter culture.