TABLE 1

Inhibition or augmentation of yeast binding of SAP and phagocytosis by human macrophages in the presence or absence of various additivesa


Treatment
% of yeasts
binding SAP
(mean ± SEM)
No. of yeasts
phagocytosed
(mean ± SEM)
% inhibition or
augmentationb
Significancec
Controld74 ± 2
10 mM EDTA67 ± 2−90.08
25 mM EDTA61 ± 1−180.005
50 mM EDTA53 ± 1−280.001
100 mM EDTA33 ± 1−55<0.001
Phagocytosis, controle136 ± 9
100 mM mannose30 ± 9−78<0.001
Phagocytosis, controle90 ± 4
CRP104 ± 4+160.024
Phagocytosis, controle112 ± 9
SAP-soaked yeast43 ± 3−620.004
Phagocytosis, controlf53 ± 7
SAP (30 µg/ml)14 ± 2−74<0.001
SAP ex serumg9 ± 1−83<0.001
  • a All values are from a minimum of three independent experiments, each with a minimum of eight replicates.

  • b Inhibition shown by negative values, and augmentation indicated by positive values.

  • c Significance is for comparison of variable with macrophages only. Significance was determined by Mann-Whitney U test or unpaired t test.

  • d Results of flow cytometry; serum, yeasts, and additive incubated for 1 h. See Fig. S1 in the supplemental material for a graphical representation of the experiment.

  • e Treatments added in serum-free medium to 96-well plates followed by the addition of yeasts, shaking at 37°C, and then counting phagocytosed cells.

  • f Results from growth of macrophages in chamber slides.

  • g SAP ex serum indicates SAP offered in serum.