TABLE 2

Self-assembly phenotypes of HIV-1 CA mutations in vitro

ProteinIn vitro assemblyPhenotypeInterpretationa
CA+ + +TubesHexamer >> pentamer
R18A+ + +Spheres, sheetsMay stabilize pentamers
E28A+ + (attenuation)Tubes, spheres, sheetsHexamer > pentamer
R18A/E28A+ (attenuation)Spheres, sheetsMay stabilize pentamers
  • a +++, efficient in vitro assembly as compared to WT CA; ++, attenuated (3-6 fold decrease) in vitro assembly as compared to WT CA; +, severely attenuated (>6 fold decrease) in vitro assembly as compared to WT CA. Tubes and sheets are assumed to be formed by hexamers; spheres are assumed to also contain pentamers.