TABLE 1

Antibiotics used in this study and their corresponding MIC values for the LTEE ancestral strain REL606

AntibioticAbbreviationaFamilyCellular targetAncestral MIC (μg/ml)
AmikacinAMKAminoglycosideProtein synthesis, binds 30S subunit4
Amoxicillin-clavulanic acidAMCβ-Lactam with β-lactamase inhibitorCell wall synthesis1
AmpicillinAMPβ-LactamCell wall synthesis2
CefotaximeCTXβ-LactamCell wall synthesis0.06
CeftriaxoneCROβ-LactamCell wall synthesis0.12
ChloramphenicolCHLOtherProtein synthesis, binds 50S subunit1
CiprofloxacinCIPFluoroquinoloneDNA synthesis0.005
ColistinCSTPolypeptideCell membranes0.125
ImipenemIPMβ-LactamCell wall synthesis0.25
LevofloxacinLVXFluoroquinoloneDNA synthesis0.015
RifampinRIFPolyketideTranscription, binds β-subunit of RNA polymerase16
StreptomycinSTRAminoglycosideProtein synthesis, binds 30S subunit128
Trimethoprim-sulfamethoxazoleSXTSulfonamideFolate synthesis0.25
TetracyclineTETTetracyclineProtein synthesis, binds 30S subunit0.5
TicarcillinTICβ-LactamCell wall synthesis4