Fluconazole-induced MTL-homozygous strains used in mating experiments

StrainParentRelevant genotypeaFluconazole
MIC (µg/ml)
SCERG11R32hom1ASCERG11R32AERG11G464S/ERG11G464S; MTLα/α; GLN3-1/GLN3-18
SCERG11R32hom1BSCERG11R32BERG11G464S/ERG11G464S; MTLa/a; GLN3-1/GLN3-28
SCMRR1R32hom1ASCMRR1R32AMRR1P683S/MRR1P683S; MTLα/α; GLN3-1/GLN3-1; CAP1-1/CAP1-216
SCMRR1R32hom2ASCMRR1R32AMRR1P683S/MRR1P683S; MTLa/a; GLN3-2/GLN3-2; CAP1-1/CAP1-216
SCTAC1R32hom2ASCTAC1R32ATAC1G980E/TAC1G980E; MTLα/α; GLN3-1/GLN3-28
SCTAC1R32hom2BSCTAC1R32BTAC1G980E/TAC1G980E; MTLa/a; GLN3-1/GLN3-28
SCUPC2R12hom1ASCUPC2R12AUPC2G648D/UPC2-2; MTLa/a; GLN3-2/GLN3-22
SCUPC2R12hom1BSCUPC2R12BUPC2G648D/UPC2-2; MTLα/α; GLN3-1/GLN3-12
  • a The two GLN3 alleles on the right arm of Chr5 in strain SC5314 were distinguished by a ClaI restriction site polymorphism; the GLN3 allele with the upstream ClaI site at position −1712 (on Chr5B) was designated GLN3-1 (67). The two CAP1 alleles on the left arm of Chr3 in strain SC5314 were distinguished by an EcoRI restriction site polymorphism; the CAP1 allele with the EcoRI site at position +1212 (on Chr3A) was designated CAP1-2 (62).