TABLE 1

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant characteristicReference
or source
S. aureus strains
    RN4220Restriction-deficient transformation recipient36
    TCH1516Community-associated USA300 MRSA isolate37
    UAMS-1Osteomyelitis clinical isolate38
    SH1000rsbU repaired laboratory strain39
    NewmanLaboratory strain40
    AH1263USA300 LAC isolate cured of plasmid LAC-p0341
    JE2USA300 LAC isolate cured of plasmids LAC-p01 and LAC-p0342
    RKC0602UAMS-1 pMK4This work
    RKC0603UAMS-1 pMK4_Teg41This work
    RKC0600SH1000 pMK4This work
    RKC0601SH1000 pMK4_Teg41This work
    RKC0535AH1263 pMK4_Teg41This work
    RKC0604Newman pMK4This work
    RKC0605Newman pMK4_Teg41This work
    RKC0072TCH1516 pMK4This work
    RKC0474TCH1516 pMK4_Teg41This work
    RKC0494JE2 pMK4This work
    RKC0470JE2 pMK4_Teg41This work
    RKC0599AH1263 pMK4This work
    RKC0538AH1263 pMK4_Teg41This work
    RKC0630AH1263 pCN51This work
    RKC0614AH1263 pCN51_Teg41This work
    RKC0628AH1263 pCN51_Teg41_3′This work
    RKC0552AH1263 pMK4_Teg41_5′This work
    NE1354USA300 JE2 hla::Bursa, hla NARSA transposon mutant42
    RKC0183TCH1516 hla::Bursa, hla mutantThis work
    RKC0472TCH1516 hla::Bursa pMK4_Teg41This work
    RKC0521AH1263 hla::Bursa, hla mutantThis work
    BB2373JE2 ΔpsmαB. Boles
    RKC0504JE2 Δpsmα pMK4This work
    RKC0442JE2 Δpsmα pMK4_Teg41This work
    JLB162AH1263 Teg41 3′ deletion (Teg41Δ3′)This work
    RKC0535JLB162 pMK4_Teg41This work
    RKC0553JLB162 pMK4_Teg41_5′This work
    RKC0631JLB162 pCN51This work
    RKC0615JLB162 pCN51_Teg41This work
    RKC0629JLB162 pCN51_Teg41_3′This work
S. epidermidis strains
    1457Wild-type S. epidermidis43
    RKC05181457 pMK4This work
    RKC05191457 pMK4_Teg41This work
E. coli strain
    DH5αCloning strainInvitrogen
Plasmids
    pMK4Gram-positive shuttle vector (Cmr)44
    pRKC0486pMK4_Teg41 (vector overexpressing Teg41 from its native promoter)This work
    pRKC0554pMK4_Teg41_5′ (vector overexpressing the 5′end of Teg41 from its native promoter,
i.e., Teg41 missing the 3′ end)
This work
    pCN51Cadmium-inducible expression vector45
    pRKC0473pCN51_Teg41 (vector overexpressing Teg41 from an inducible promoter)This work
    pRKC0628pCN51_Teg41_3′(vector overexpressing the 3′ end of Teg41 from an inducible promoter)This work
    pJB38Temperature-sensitive allelic exchange plasmid (Cmr)46
    pJB1037pJB38 containing fragment upstream of Teg41This work
    pJB1039pJB1037 containing fragment downstream of Teg41This work