TABLE 3

Primers and probes used in this studya

Assay componentNameSequence
Real-time PCR primerEVD68_F1CRTGGGTCTTCCTGACTTRAC
EVD68_F2AYRGGCCTTCCTGACTTGAC
EVD68_F3YGTGGGTCTTCCTGACTTGAC
EVD68_R1RCCTGAYTGCCARTGGAATG
EVD68_R2GCCTGAYTGCCARTGGAAYG
Real-time PCR probeEVD68_FB16FAM-CARGCAATGTTTGTACCBACTGGTGC-BHQ
EVD68_FB26FAM-CAAGCAATGTTYGTRCCCACTGGTGC-BHQ
Amplicon sequencing primerEVD68-UT_F1ACCCAACTGAATGGAGCCRTGGGTCTTCCTGACTTRAC
EVD68-UT_F2ACCCAACTGAATGGAGCAYRGGCCTTCCTGACTTGAC
EVD68-UT_F3ACCCAACTGAATGGAGCYGTGGGTCTTCCTGACTTGAC
EVD68-UT_R1ACGCACTTGACTTGTCTTCRCCTGAYTGCCARTGGAATG
EVD68-UT_R2ACGCACTTGACTTGTCTTCGCCTGAYTGCCARTGGAAYG
16S amplicon sequencing primerUT1-S-D-Bact-0341-b-S-17bACCCAACTGAATGGAGCCCTACGGGNGGCWGCAG
UT2-S-D-Bact-0785-a-A-21bACGCACTTGACTTGTCTTCGACTACHVGGGTATCTAATCC
  • a The EV-D68 assays target the VP1 gene, and the real-time PCR results in a 94-bp amplicon. Underlined sequences are the universal tails. 6FAM, 6-carboxyfluorescein; BHQ, black hole quencher.

  • b Primers without these universal tails are from reference 70.