TABLE 3

Primers and plasmids used in this study

Oligonucleotide or plasmidSequence (5′–3′) or notesReference
Oligonucleotides
    Quantitative real-time PCR
        agrB FWDGACCAGTTTGCCACGTATCTThis study
        agrB REVGCTAAGACCTGCATCCCTAATCThis study
        RNAIII FWDGGAGTGATTTCAATGGCACAAGThis study
        RNAIII REVGTGAATTTGTTCACTGTGTCGATAAThis study
        psmα1 FWDGGTATCATCGCTGGCATCATTThis study
        psmα1 REVCCATGTGAAAGACCTCCTTTGTThis study
        pmtA FWDCTTGCGCATGTTCTGTTAATCCThis study
        pmtA REVTCAAGTTGTGAGTGGTGCTATTThis study
        16S rRNA FWDGTGGAGGGTCATTGGAAACTThis study
        16S rRNA REVCACTGGTGTTCCTCCATATCTCThis study
    agrC complementation
        agrC FWD BamHITGGATCCAGAGGAGAAATTAAATGATThis study
        agrC REV SstICCCGAGCTCCTAGTTGTTAATAATTTCAACThis study
    Transposon insertion sequencing
        RP45GAGTGTGATGATAAGTGGGAAGGACThis study
        SS1905GGTAAACTATGATTCACGACGACTAGThis study
Plasmids
    pCL15E. coli-S. aureus shuttle vector70
    pCL15-Pspac-agrCagrC complementation vectorThis study
    pTXΔpsmα1-4psm complementation vector39
    pTXΔ16Empty vector81