TABLE 2

Functionality of the l-arginine biosynthesis pathwaya

GeneSizeb in
E. coli
Size in
S. praecaptivus
ReactionS. glossinidiuscConclusion
argA1,332 bpSant_0864ACGSSGGMMB4_04654 (argA_1)argA_2 may produce a subunit that can function
individually, using its GTG start codon
443 aa418 aaSGGMMB4_04655 (argA_2)
804 bp total
argB777 bpSant_3956ACGKSGGMMB4_05193New annotation indicates full-length gene
258 aa257 aa765 bp
argC1,005 bpSant_3957AGPRSGGMMB4_05194New annotation indicates full-length gene
and ArgC detected by proteomics
334 aa334 aa999 bp
gabT1,281 bpSant_2160ACOTASee argDSee argD
426 aa425 aa
argD1,221 bpSant_0398ACOTASGGMMB4_05438 (argD_1)
1–708 bp
May use functional alternatives bioA
(SG0902) or hemL (SG0500)
406 aa407 aaSGGMMB4_05439 (argD_2)
681–828 bp
argE1,152 bpSant_3958ACODASGGMMB4_05195New annotation indicates full-length gene
383 aa382 aa1,146 bp
argF1,005 bpSant_3829OCBTSGGMMB4_05057 New annotation indicates full-length gene
334 aa338 aa1,014 bp
argI1,005 bpSant_3829OCBTSGGMMB4_05057 New annotation indicates full-length gene
334 aa338 aa1,014 bp
argG1,344 bpSant_2433ARGSSSGGMMB4_03589 (argG_1)New annotation indicates argG_2 fragment
is almost full length
447 aa445 aaSGGMMB4_03590 (argG_2)
1,341 bp total
argH1,374 bpSant_3955ARGSLSGGMMB4_05192New annotation indicates full-length gene
457 aa457 aa1,371 bp
  • a tBLASTn results for S. glossinidius orthologues of components of the l-arginine biosynthesis pathway in E. coli and S. praecaptivus.

  • b aa, amino acids.

  • c Functional orthologues are indicated by italic boldface type, and those components for which the new S. glossinidius genome annotation has provided evidence for functionality are shown in italic type.