TABLE 1

S. aureus isolates and strains used in studya

StrainBackgroundDescriptionReference or source
VJT 12.61USA300WT LAC60
VJT 20.06USA300WT FPR375761
VJT 15.36USA300WT BK1880725
VJT 10.21USA500WT BK239562
VJT 1.01NewmanWT Newman MSSA20
VJT 15.46ST239WT BK23604 (BS991)Shopsin laboratory
VJT 21.06USA800WT NRS387NARSA (25)
VJT 5.81CC8WT BK4645b MSSA63
VJT 4.79USA400WT MW2 CA-MRSA64
VJT 50.06CC1WT MSSA47665
VJT 50.03CC5WT N315 HA-MRSA66
VJT 50.04USA700WT 502A67
VJT 21.02USA100WT NRS382NARSA
VJT 23.05USA100WT MSSA#1640
VJT 35.72USA100WT NRS786NARSA
VJT 35.74USA100WT NRS788NARSA
VJT 38.02USA100WT MSSA#2740
VJT 15.44CC30WT BK22820 phage-type 80/81 pandemic strain (BS992)Shopsin laboratory
VJT 50.09CC30WT ATCC 25923ATCC
VJT 2.59USA200WT UAMS-168
VJT 21.08USA1100WT NRS484NARSA (25)
VJT 38.11USA600WT MSSA#3740
VJT 14.26USA300ΔlukAB LAC20
VJT 16.39USA300ΔlukAB BK1880720
VJT 11.39USA400ΔlukAB MW220
VJT 22.31NewmanΔlukAB Newman20
VJT 11.36CC8ΔlukAB BK4645b20
VJT 15.78USA300 LACWT (AH1263)69
VJT 47.15USA300 LAChlgACB::tet lukED::kan pvl::spec ΔlukAB (ΔΔΔΔΔ USA300)70
VJT 44.10USA300 LACpvl::spec71
VJT 30.01USA300 LAClukED::kan71
VJT 29.98USA300 LAChlgACB::tet70
VJT 23.52USA300 LACΔlukAB11
VJT 38.84USA300 LAChlgACB::tet lukED::kan pvl::spec70
VJT 49.33USA300 LACWT pOS1-PsarA-SOD-RBS-sGFP (Cmr)This study
VJT 49.34USA300 LAChlgACB::tet lukED::kan pvl::spec ΔlukAB pOS1-PsarA-SOD-RBS-sGFP (Cmr)This study
VJT 49.35USA300 LACΔlukAB pOS1-PsarA-SOD-RBS-sGFP (Cmr)This study
VJT 49.36USA300 LAChlgACB::tet lukED::kan pvl::spec pOS1-PsarA-SOD-RBS-sGFP (Cmr)This study
VJT 26.87USA300 LACpXEN1-lux, luciferase reporter promoterless control (Cmr)11
VJT 26.89USA300 LACpXEN1-plukAB-lux, lukAB promoter driving expression of lux operon (Cmr)11
VJT 26.91USA300 LACpXEN1-plukSF-PV-lux, pvl promoter driving expression of lux operon (Cmr)11
VJT 26.92USA300 LACpXEN1-phlgCB-lux, hlgCB promoter driving expression of lux operon (Cmr)11
VJT 26.93USA300 LACpXEN1-phlgA-lux, hlgA promoter driving expression of lux operon (Cmr)11
VJT 26.95USA300 LACpXEN1-plukED-lux, lukED promoter driving expression of lux operon (Cmr)11
VJT 31.57NewmanΔlukED ΔhlgACB::tet ΔlukAB::spec Δhla::ermC (ΔΔΔΔΔ Newman)59
  • a WT, wild type; HA-MRSA, hospital-acquired S. aureus; Cmr, chloramphenicol resistance; NARSA, Network on Antimicrobial Resistance in S. aureus; ATCC, American Type Culture Collection.