TABLE 1

Thermodynamic parameters for the binding of ligands to McpU-LBD and TlpQ-LBD as derived from ITC experimentsa

CompoundMcpU-LBDTlpQ-LBDKD McpU-LBD/KD TlpQ-LBD
KD (µM)ΔH (kcal · mol−1)KD (nM)ΔH (kcal · mol−1)
Putrescine2 ± 0.1b−15 ± 0.5134 ± 12−6.8 ± 0.315
Cadaverine22 ± 2b−15.5 ± 0.5150 ± 4−6.0 ± 0.1147
Spermidine4.5 ± 0.4b−4.3 ± 0.356 ± 4−4.6± 0.480
Agmatine0.48 ± 0.02−14.5 ± 0.2150 ± 9−5.4 ± 0.13
Ethylenediamine39 ± 4−9.7 ± 0.51,710 ± 180−6.3 ± 0.623
Histamine26 ± 2−2.6 ± 0.3639 ± 27−9.1 ± 0.341
  • a Means and standard deviations represent data from three independent experiments.

  • b Reported previously in reference 20.