TABLE 1 

Crystallographic data

ParameterValue(s) for E. coli periplasmic FtsQB complex
AloneSeMetFtsB peptide
NCBI database IDsFtsQ: FTSQ_ECOLIFtsQ: FTSQ_ECOLIFtsQ: FTSQ_ECOLI
FtsB: FTSB_ECOLIFtsB: FTSB_ECOLIFtsB: FTSB_ECOLI
ConstructsQ: M-58-276-GSH6Q: M-58-276-GSH6Q: M-58-276-GSH6
B: pwdB: MGSSHHHHHHSSGLVPRGSHM-22-103B: 64–87 (synthetic, acetylated,
M77 replaced with norleucin)
-22-103RGSHM-22-103
Method Molecular replacementSelenium SADMolecular replacement
Data collection statistics
    Beamline/sourceDiamond I04-1Diamond I03Diamond I04
    Wavelength (Å)0.928190.979800.97950
Crystal/helical
    Space/point groupP41212P41212C2
    Cell (Å)93.8, 93.8, 106.492.5, 92.5, 105.2117.2, 48.0, 131.6, 112.4°
Data
    Resolution (Å)2.62.82.8
    Completeness (%)a99.5 (99.5)100.0 (99.9)98.5 (99.7)
    Multiplicitya12.8 (13.4)14.0 (13.8)3.2 (3.3)
    (I)/σ(I)a22.6 (2.1)33.3 (3.9)10.6 (1.8)
    Rmergea0.082 (1.702)0.078 (1.130)0.077 (0.744)
    Rpima0.024 (0.477)0.021 (0.314)0.051 (0.488)
    CC1/20.999 (0.821)1.000 (0.944)0.998 (0.686)
    Anomalous correlation 0.833 (0.010)
    Selenium sites1
Refinement statistics
    R/Rfreeb0.2228/0.25190.2299/0.2770
    
    ModelsFtsQ 58–2602× FtsQ 58–260
    FtsB 64–872× FtsB 64–87
    7 waters0 waters
    Bond length RMSD (Å)c0.0090.012
    Bond angle RMSD (°)1.1621.637
    Favored (%)d99.599.5
    Disallowed (%)d0.0 0.5
    MOLPROBITY score 97th percentile98th percentile
PDB IDs 6H9N6H9O
  • a Values in parentheses for these parameters refer to the highest recorded resolution shell.

  • b Five percent of the reflections were randomly selected before refinement.

  • c RMSD, root mean square deviation.

  • d Percentage of residues in the Ramachandran plot (PROCHECK “most favored” and “additionally allowed” added together).