TABLE 1 

MS7163 genes required for curli production

Gene nameNo. of insertsNo. of readsCongo red bindingaCsgA expression by defined mutantaProductb
Tn5
mutants
Defined
mutants
Known curli regulators
    dksA24,496NDNDRNA polymerase-binding transcription factor
    ihfB49,172NDNDIntegration host factor subunit beta
    rne12,276NDNDRNase E
    ihfA12,120NDNDIntegration host factor subunit alpha
    mlrA927,691NDNDHTH-type transcriptional regulator
    ompR13,126NDNDTranscriptional regulatory protein
Curli genes
    csgG1144,759NDNDCurli secretion channel
    csgB12,441NDNDCurli minor subunit
    csgA39,637NDNDCurli major subunit
Purine de novo biosynthesis
    purK12,029N5-Carboxyaminoimidazole ribonucleotide synthase
    purF26,421Amidophosphoribosyl transferase
    purM26,906Phosphoribosylformylglycinamidine cyclo-ligase
    purL411,161NDNDPhosphoribosylformylglycinamide synthase
    purD11,422Phosphoribosylamine-glycine ligase
    purH26,899NDNDAICAR transformylase/IMP cyclohydrolase
Intergenic regions
    yccT-yccU25,164NDNDIntergenic region between yccT and yccU
    nudE-yrfF25,713NDNDIntergenic region between nudE and yrfF
    rcpA-gadW311,579Upstream of rcpA and downstream of gadW
Others
    yrfF717,067Putative membrane protein
    kdgK_226,169++2-Dehydro-3-deoxygluconokinase
    rcsD515,895++Phosphotransferase
    wzyE13,302++ECA polysaccharide chain elongation
  • a −, negative phenotype; +, positive phenotype; ND, not done.

  • b HTH, helix-turn-helix; AICAR, 5-aminoimidazole-4-carboxamide ribonucleotide; ECA, enterobacterial common antigen.