TABLE 1 

Identification of selected proteins from 2-D DIGE of B. burgdorferi (pH 3 to 10)a

Spot no.PER, expt 1/expt 2Top-ranked protein (species)Accession no.Protein MWProtein pI
5−2.75/−3.77Integral OMP p66 (B. burgdorferi B31)gi|1559494868,1306.04
6−3.02/−3.64Membrane protein (B. burgdorferi)gi|70032399068,1306.04
7−2.94/−3.41Membrane protein (B. burgdorferi)gi|70032399068,1306.04
8−1.45/−2.05Integral OMP p66 (B. burgdorferi B31)gi|1559494868,1306.04
9−1.68/−2.06ABC TSBP (B. burgdorferi)gi|70032373962,3299.16
13−1.55/1.27Oligoendopeptidase F (B. burgdorferi)gi|49919217669,6796.84
15−1.23/−2.79ABC TSBP (B. burgdorferi)gi|70032373962,3299.16
16−2.08/−1.95Extracellular SBP (B. burgdorferi)gi|48268127562,3199.12
19−2.80/−3.07Glycerol kinasegi|668546255,5717.02
20−2.74/−3.53Glycerol kinasegi|668546255,5717.02
21−1.96/−2.33Glycerol kinasegi|668546255,5717.02
24−1.74/−2.42Glycerol kinasegi|668546255,5717.02
25−2.63/−3.08GPDH (B. burgdorferi)gi|48031362054,4788.89
26−2.51/−2.89GPDH (B. burgdorferi)gi|48031365855,2238.88
38−2.33/−1.31GAPDH (B. burgdorferi)gi|14674362234,9656.79
39−2.17/−1.13GAPDHgi|391570236,2327.74
46−1.74/−1.77Chain E, Lyme disease antigen OspAgi|1151469127,6238.35
48−1.83/−1.87Putative lipoprotein (B. burgdorferi ZS7)gi|21816457736,4398.88
51−2.20/−2.56Chain E, Lyme disease antigen OspAgi|1151469127,6238.35
73−1.99/−4.76Peptide deformylase (B. burgdorferi)gi|48873864919,1046.32
75−1.53/−1.92NDPK (B. burgdorferi)gi|48862032419,3376.52
82−1.83/−2.89Integral OMP p66 (B. burgdorferi B31)gi|1559494868,1306.04
83−2.42/−3.27Integral OMP p66 (B. burgdorferi B31)gi|1559494868,1306.04
85−2.43/−3.64Integral OMP, partial (B. burgdorferi)gi|69799621026,2336.04
86−2.14/−2.13Chain E, Lyme disease antigen OspAgi|1151469127,6238.35
87−2.81/−4.11Antigen, p83/100 (B. burgdorferi Bol26)gi|22623323779,3245.09
89−1.99/−2.60P41, partial (B. burgdorferi)gi|48360335,7305.54
  • a Abbreviations: PER, protein expression ratio; OMP, outer membrane protein; TSBP, transporter substrate-binding protein; SBP, solute-binding protein; GPDH, glycerol 3-phosphate dehydrogenase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; NDPK, nucleoside-diphosphate kinase.