TABLE 4 

TpCM-2a

ComponentAmt for 50 mlFinal concnManufacturer/catalog no.
1× CMRL 1066 medium without l-glutamine or phenol red37 ml0.8×United States Biological/C5900-03A
Sodium pyruvate364 µl0.73 mMSigma/S8636
0.1% resazurin50 µl0.001%Sigma/R7017
MOPS (1 M), pH 7.51 ml20 mMSigma/M3183
NaHCO3 (7.5%)1.08 ml19.2 mMSigma/M8761
l-Glutamine (200 mM)500 µl2 mMSigma/G6152
100× d-glucose (15% in water)500 µlTo 17.6 mMSigma/G6152
d-Mannitol (10 g/dl) (10% in water)80 µl0.88 mMSigma/M-1902
l-Histidine (5 g/dl) (5% in water)80 µl0.52 mMSigma/H6034
dl-Dithiothreitol4 mg0.52 mMSigma/D9779
Fetal bovine serum, heat inactivated10 ml20%Sigma/F4135
  • a TpCM-2, T. pallidum cultivation medium 2; MOPS, morpholinepropanesulfonic acid.