TABLE 1 

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant genotypeSource or reference
Strains
    M. tuberculosis
        H37RvWild type (WT)ATCC 25618
        MHD18Hygr WT with pMV3063
        MHD761Strepr WT with pMV306.strep8
        MHD794Kanr WT with pMV306.kan44
        MHD1033Kanr Rv0077c::MycoMarT7 with transposon insertion between codons 64 and 65This work
        MHD1077Kanr Strepr MHD1033 with pMV306.strep-Rv0077cThis work
        MHD1086Kanr Strepr MHD1033 with pMV306.strepThis work
        MHD1293Hygr ΔRv0078::hygThis work
        MHD1315Hygr Kanr ΔRv0078::hyg with pMV306.kanThis work
        MHD1316Hygr Kanr ΔRv0078::hyg with pMV306.kan-Rv0078W100RThis work
        MHD1318Hygr Kanr ΔRv0078::hyg with pMV306.kan-Rv0078This work
    E. coli
        ER2566F λ fhuA2 [lon] ompT lacZ::T7 geneI gal sulA11 Δ(mcrC-mrr)114::IS10R(mcr-73::miniTn10)
2R(zgb-210::Tn10)1 (Tets) endA1 [dcm]
45
        DH5αF φ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17 (rK mK+)
phoA supE44 λ thi-1 gyrA96 relA1
Gibco, BRL
 
Plasmids
    pET24b(+)Kanr plasmid for production of C-terminal His6 epitope-tagged proteinNovagen
    pMV306.kanKanr plasmid that integrates at attB site on M. tuberculosis chromosome46
    pMV306Hygr plasmid that integrates at attB site on M. tuberculosis chromosome46
    pMV306.strepStrepr plasmid that integrates at attB site on M. tuberculosis chromosomeGift from
John Mckinney
    pYUB854Hygr allelic exchange vector34
    pMV306.strep-Rv0077cStrepr pMV306.strep plasmid with Rv0077c and 200 bp upstream of start codon of Rv0077cThis work
    pMV306.kan-Rv0078Kanr pMV306.kan plasmid with Rv0078 and 200 bp upstream of start codon of Rv0078This work
    pMV306.kan-Rv0078W100RKanr pMV306.kan-Rv0078 plasmid with Trp100 changed to ArgThis work
    pET24b(+)-Rv0077cHis6Kanr; for production of Rv0077c-His6 in E. coliThis work
    pET24b(+)-Rv0078His6Kanr plasmid for production of Rv0078-His6 in E. coliThis work