TABLE 2 

Production of xanthocillin derivatives in liquid shake culture (compounds 7 to 12), comparing the OE::xanC mutant to deletion strains

CompoundProduction of derivative ina:
OE::xanC mutantWTDeletion mutant
−Cu+Cu−Cu+CuΔcrmAΔcrmCDΔxanBΔxanB ΔcrmA
74.4E8 ± 2.1E81.7E9 ± 1.4E85.5E6 ± 2.2E61.9E6 ± 9.1E56.1E6 ± 2.5E61.8E6 ± 1.0E600
82.6E9 ± 8.7E84.6E9 ± 5.1E84.9E7 ± 9.0E65.1E6 ± 2.1E65.3E7 ± 2.8E75.9E7 ± 8.3E600
91.3E9 ± 4.3E81.3E9 ± 9.9E72.0E7 ± 1.8E61.1E6 ± 4.6E51.9E7 ± 9.7E62.9E7 ± 3.6E600
104.6E8 ± 2.2E84.3E9 ± 3.9E85.6E6 ± 1.3E61.5E6 ± 5.5E52.7E6 ± 1.4E61.4E6 ± 4.0E500
1108.4E7 ± 2.6E7000000
1209.9E6 ± 2.7E6000000
  • a The numbers indicate the average ± standard deviation for the peak intensity of each compound, as determined by LC-HRMS with four replicates. All deletion mutants were grown under copper-depleted conditions.