TABLE 1 

Generation times of deletion strains in mucin-derived sugars

Sugar conditionAvg generation time (min) ± SD
WTΔkdpEΔcraΔfusRΔkdpE ΔcraΔfusR ΔcraΔfusR ΔkdpEΔfusR ΔkdpE Δcra
0.1% glucose90.5 ± 16.0101.2 ± 12.685.9 ± 15.3106.3 ± 14.067.0 ± 0.190.2 ± 9.178.2 ± 12.590.5 ± 2.2
0.4% glucose78.2 ± 4.3116.8 ± 29.175.0 ± 12.7120.1 ± 37.571.4 ± 1.2132.3 ± 9.0a74.2 ± 20.5102.2 ± 9.63
0.4% galactose134.9 ± 23.9199.6 ± 39.692.0 ± 28.9197.0 ± 7.0219.2 ± 9.2183.7 ± 6.2152.7 ± 21.3164.9 ± 6.4
0.4% fructose168.9 ± 24.9211.0 ± 12.0148.6 ± 3.6233.4 ± 6.9150.5 ± 26.6191.0 ± 12.7160.7 ± 31.6169.5 ± 5.5
0.4% mannose115.6 ± 5.5113.5 ± 3.1104.3 ± 25.7137.6 ± 18.082.4 ± 13.9124.4 ± 26.8108.0 ± 7.9116.8 ± 19.3
0.4% N-acetylglucosamine113.7 ± 12.7174.2 ± 6.0130.2 ± 8.8184.4 ± 2.7164.9 ± 7.1146.9 ± 28.5134.4 ± 10.2139.0 ± 19.1
0.4% N-acetylgalactosamine136.4 ± 23.0170.3 ± 34.5196.7 ± 66.1153.3 ± 7.9135.6 ± 40.7175.6 ± 41.5119.6 ± 11.3253.2 ± 29.6
0.4% sialic acid109.7 ± 8.9131.8 ± 15.3139.3 ± 7.9148.1 ± 13.0121.6 ± 7.7144.0 ± 36.6116.2 ± 38.9145.1 ± 28.2
0.4% rhamnose137.3 ± 4.5174.1 ± 21.3133.4 ± 21.4151.6 ± 28.598.0 ± 10.7103.2 ± 3.3a106.3 ± 7.0a121.5 ± 12.8
0.4% fucose125.6 ± 48.1186.2 ± 0.9270.3 ± 142.2138.7 ± 18.8138.7 ± 18.8167.5 ± 31.7130.6 ± 20.0204.6 ± 30.1
0.4% pyruvate138.2 ± 12.7172.3 ± 10.5None171.9 ± 9.3NoneNone109.0 ± 12.2None
0.4% galacturonic acid45.6 ± 0.759.9 ± 4.167.7 ± 7.652.1 ± 4.878.5 ± 11.4103.4 ± 1.7a52.8 ± 8.4107.0 ± 3.7a
0.4% gluconic acid108.9 ± 18.1187.3 ± 13.5a142.8 ± 24.0157.0 ± 25.0103.1 ± 36.4123.2 ± 9.0119.0 ± 5.4164.1 ± 7.8
0.4% glucuronic acid120.9 ± 23.9187.3 ± 13.5132.5 ± 38.5150.4 ± 46.2103.7 ± 35.5121.3 ± 11.6119.0 ± 5.4153.1 ± 15.8
0.4% arabinose160.6 ± 13.4217.4 ± 11.9a126.4 ± 10.8204.6 ± 4.5167.7 ± 31.1135.9 ± 28.5154.0 ± 32.5128.3 ± 9.1
0.4% xylose103.2 ± 18.4130.5 ± 16.687.6 ± 11.6168.6 ± 25.7108.9 ± 50.3104.7 ± 31.0120.6 ± 66.5122.4 ± 29.3
0.4% ribose116.7 ± 2.2198.1 ± 1.6a217.5 ± 7.3a185.0 ± 6.0a195.6 ± 16.0258.0 ± 24.8176.3 ± 15.796.6 ± 12.3
  • a Significantly different (P > 0.05, Student’s t test) from the WT grown with the sugar indicated.