TABLE 1 

Description and treatment of black-capped chickadees, red-breasted nuthatches, and Northwestern crows sampled

Species and specimenaYrAKD statusPoecivirus presentBeak length (mm)Beak appearance, histopathologyNGSbSample processing notes
ElongatedCrossedNormal, cellular-level hyperkeratosis
BCCH
    1602001AffectedYes9.3XYesFull Sanger sequencing of ORF
    2632001AffectedYes9.3XYes
    3942001AffectedYes9.4XYesFull Sanger sequencing of ORF
    4492002AffectedYes9.5XYesFull Sanger sequencing of ORF
    4512002AffectedYes11XYes
    4782003AffectedYes21.1XXYes
    4982004AffectedYes40.3XXYesFull Sanger sequencing of ORF
    6002006AffectedYes8.1XYes
    7382010AffectedYes8XFull Sanger sequencing of ORF
    9532014AffectedYes25.1XXStored at 4°C overnight prior to processing for poecivirus detection using virus-specific primers
    9542014AffectedYes25.1XStored at 4°C overnight prior to processing for poecivirus detection using virus-specific primers
    9552014AffectedYes23.1XXPoecivirus detection using virus-specific primers
    9562014AffectedYes8.7XStored at 4°C overnight prior to processing for poecivirus detection using virus-specific primers
    9672015AffectedYes19.6XXStored at 4°C overnight prior to processing for poecivirus detection using virus-specific primers
    9682015AffectedYes9.5XXStored at 4°C overnight prior to processing for poecivirus detection using virus-specific primers
    9702015AffectedYes8.8XStored at 4°C overnight prior to processing for poecivirus detection using virus-specific primers
    9712015AffectedYes18.7XXStored at 4°C overnight prior to processing for poecivirus detection using virus-specific primers
    9722015AffectedYes15.7XXPoecivirus detection using virus-specific primers
    9732015AffectedYes9.0XXPoecivirus detection using virus-specific primers
    1831995UnaffectedNo7.3Poecivirus detection using virus-specific primers
    3892001UnaffectedNo7Poecivirus detection using virus-specific primers
    3922001UnaffectedNo7.3Poecivirus detection using virus-specific primers
    4012001UnaffectedNo7.3Poecivirus detection using virus-specific primers
    4602003UnaffectedNo7.3Poecivirus detection using virus-specific primers
    4732003UnaffectedNo7.3Poecivirus detection using virus-specific primers
    5962007UnaffectedYes7Full Sanger sequencing of ORF
    6012008UnaffectedYes7.4Full Sanger sequencing of ORF
    7392010UnaffectedNo7.3Poecivirus detection using virus-specific primers
RBNH
    121999AffectedYes12.1XXPoecivirus detection using virus-specific primers
    9292012AffectedYes29.6XXPoecivirus detection using virus-specific primers
NOCR
    6742005AffectedYes36.3XPoecivirus detection using virus-specific primers
    8482007AffectedYes33.1XXPoecivirus detection using virus-specific primers
  • a BCCH, black-capped chickadees; RBNH, red-breasted nuthatches; NOCR, northwestern crows.

  • b NGS, next-generation sequencing.