TABLE 3 

Predicted ORFs in cyanophage N-1 with high similarity to cyanobacterial genes

ORFLength (bp)StrandaSignificant hitOrganism (accession no.)E value% Identity (no.
of shared aa)b
11,359FPhage terminase, large subunitNostoc sp. strain PCC 75242.32e−5531 (134)
21,602FHypothetical proteinNostoc sp. strain PCC 7524 (AFY48995)3.55e−1822 (106)
6330FHypothetical proteinNostoc sp. strain PCC 7524 (AFY49001)9.62e−0630 (30)
91,521FTail sheath proteinNostoc sp. strain PCC 7524 (AFY49006)3.74e−7439 (138)
152,694RLysozyme-like domain, rare lipoprotein A
(RlpA)-like double psi beta barrel
Nostoc sp. strain PCC 7524 (AFY49014)1.48e−3728 (137)
18849RHypothetical proteinNostoc sp. strain PCC 7524 (AFY49010)9.82e−1027.9 (51)
19918FExonuclease RNase T and DNA Pol IIIThauera sp. strain MZIT4.14e−0428.4 (38)
29330FLysozymeNostoc sp. strain PCC 7524 (AFY49017)2.51e−0324 (32)
301,167FBaseplate J phage tail proteinNostoc sp. strain PCC 7524 (AFY49018)4.18e−6843 (144)
31576FPhage tail proteinNostoc sp. strain PCC 7524 (AFY49020)2.32E+0026.3 (45)
321,395FPhage tail fiber proteinNostoc sp. strain PCC 7524 (AFY49021)2.97e−6437 (172)
331,155FTail collar proteinNostoc sp. strain PCC 7524 (AFY49022)1.62e−4733 (137)
341,896RDNA Pol BCyanothece sp. strain PCC 74247.0e−11841.5 (243)
48600RHypothetical proteinNostoc punctiforme PCC 731021.10e−0621.4 (40)
52633RC-5 cytosine-specific DNA methylaseNostoc punctiforme PCC 731021.81e−0536.2 (34)
56240RASCH domain proteinClostridium phage phiMMP024.06e−1242.3 (33)
62627FThymidylate kinaseLyngbya sp. strain PCC 81062.45e−2336 (74)
681,047FDNA-cytosine methyltransferaseAnabaena variabilis ATCC 294131.02e−5633.2(127)
75579RHypothetical proteinThermoanaerobacter italicus Ab98.67e−0332.7 (33)
76507RHypothetical proteinCalothrix sp. strain PCC 71031.73e−4756 (94)
77726RHypothetical proteinCalothrix sp. strain PCC 71034.94e−0630.4 (78)
79585FdCTP deaminaseSynechococcus sp. strain PCC 73351.45e−6965.5 (131)
  • a F, forward; R, reverse.

  • b aa, amino acids.