TABLE 1 

HGT recombination blocks separating GAS emm89/M89 clades

BlockCladesStartaStopaLength
(kb)
SNPsGenesM-like% ID
RB1bC1-C292,389164,16271,77441172M288.74b
RB2C1-C2295,481297,5742,09492M2100.00
RB3cC1-C2773,487780,6347,148558M299.55
RB4cC1-C2794,417800,6596,243288M299.55
RB5C1-C2921,261960,29739,03710041M299.68
RB6C1-C21,022,6191,030,4077,789206M299.97
RB7C1-C21,543,6511,561,16517,51510313M297.17
RB8C1-C21,577,9161,597,44719,53213821M2899.58
RB9C2-C386,60388,3661,764122M5/M2399.38
RB10C2-C3145,163155,56910,4075911M1/M1298.52
RB11C2-C3244,407244,75835251M5100.00
RB12C2-C31,472,2621,473,02576491M12100.00
RB13C2-C31,558,8981,559,69880172M4999.75
RB14C2-C31,693,6131,694,8051,19362M5/M6100.00
RB15C3-SC3D341,762359,57917,81810621M199.67
  • a The start and stop positions provided are relative to the MGAS23530 genome.

  • b The first 18.6 kb and last 39.6 kb are M2-like (>99% identity [ID]); however, the central 13.5 kb FCT pilus-encoding region is unlike that of any other sequenced GAS emm-type.

  • c RB3 and RB4 likely represent a single HGT event that encompasses the intervening streptin lantibiotic synthesis genes, thus resulting in a larger single recombination of 26,697 bp.