TABLE 4 

Interactions of FKBP12, FKBP12 variants, and the FRB domain determined by Y2H analysis

AD-gene fusionBD-gene fusionInteractionDrugb
N terminia
    FKBP12FRBRapamycin
    FKBP12FRB
    FKBP12(P90G)FRB+Rapamycin
    FKBP12(P90G)FRB
    FKBP12(V91C)FRBRapamycin
    FKBP12(V91C)FRB
    FRBFKBP12+Rapamycin
    FRBFKBP12
    FRBFKBP12(P90G)+Rapamycin
    FRBFKBP12(P90G)
    FRBFKBP12(V91C)+Rapamycin
    FRBFKBP12(V91C)
C terminic
    FKBP12FRB+Rapamycin
    FKBP12FRB
    FKBP12(P90G)FRB+Rapamycin
    FKBP12(P90G)FRB
    FKBP12(V91C)FRB+Rapamycin
    FKBP12(V91C)FRB
    FKBP12FKBP12Rapamycin
    FKBP12FKBP12FK506
    FKBP12FKBP12
    FKBP12(P90G)FKBP12(P90G)Rapamycin
    FKBP12(P90G)FKBP12(P90G)FK506
    FKBP12(P90G)FKBP12(P90G)
    FKBP12(V91C)FKBP12(V91C)Rapamycin
    FKBP12(V91C)FKBP12(V91C)FK506
    FKBP12(V91C)FKBP12(V91C)
  • a N termini of FKBP12s were fused with the GAL4 AD.

  • b Both rapamycin and FK506 were used at 1 µg/ml.

  • c C termini of FKBP12s were fused with the GAL4 AD.