TABLE 1 

Clade-specific SNPs identified between clades B and C

SNP position in EC958SNP identified in cladea:ImpactbLocus tagCodonGeneProduct
BB0C0C
H17G216JJ1897C001JJ2244G213CD306
1244787CCCCCCCCTI→VEC958_132223potBSpermidine-putrescine transport system permease
2715324GGGGGGGGASynEC958_5020125Hypothetical protein
3536312CCCCCCCCT
3555316CCCCCCCCTSynEC958_351139yhaKHypothetical protein
3921426GGGGGGGGAK→REC958_388778yhiMHypothetical protein
2536291CCCCCTCCTN→DEC958_256787gyrADNA gyrase subunit A
1504736CCCCCCCGGSynEC958_1601142yciKHypothetical oxidoreductase YciK
2744500CCCCCCCTTI→VEC958_2766224eutBProbable regulatory subunit of ethanolamine ammonia-lyase
2937986AAAAAAAGG
4032999CCCCCCCTTC→REC958_3982119yiaKHypothetical oxidoreductase YiaK
1382365GGGGGGAAASynEC958_1478204hemAGlutamyl-tRNA reductase
2756734CCCCCCTTTSynEC958_2779448maeBNADP-dependent malate dehydrogenase
3883417GGGGGGAAAM→TEC958_3846231livGATP-binding component of high-affinity branched-chain amino acid transport system LivG
53758TTTTAAAAAR→SEC958_0181147kefCGlutathione-regulated potassium efflux antiporter
2705332GGGGAAAAASynEC958_5013334nupCNucleoside-transport system protein NupC
3549826AAAATTTTTF→YEC958_3501184yqjAHypothetical protein
3587935GGGGAAAAAN→DEC958_354420agaDPhosphotransferase system, N-acetylgalactosamine-specific IID component
642980CCTTTTTTTSynEC958_0717116ybdBHypothetical protein
761921GGAAAAAAASynEC958_0832150Putative C4-dicarboxylate transporter, small permease protein (DctQ-like)
1001668TTCCCCCCCSynEC958_1062115ycaPHypothetical protein
1167464CCTTTTTTTC→REC958_1238268ymdCPutative synthase
1317794GGAAAAAAASynEC958_141636ycgKProtein YcgK
1727956GGTTTTTTTS→AEC958_1806223Probable dimethyl sulfoxide reductase chain YnfF
2191491CCTTTTTTTSynEC958_2289527Putative peptide synthetase-like protein
2395814CCTTTTTTTL→PEC958_2450556Hypothetical protein
2686010TTCCCCCCC
2692404CCTTTTTTTC→REC958_5002238ypdBHypothetical protein
2706426CCTTTTTTTSynEC958_5222482yfeAHypothetical protein
3030230GGTTTTTTTK→NEC958_3037192Hypothetical protein
3798899GGAAAAAAASynEC958_3771208yhfWHypothetical protein
3802091GGTTTTTTT
3823737AAGGGGGGG
3913371GGAAAAAAAT→AEC958_3880195Putative xylulose kinase
4009795CCTTTTTTTV→AEC958_3959327yiaEPutative dehydrogenase
4216222CCTTTTTTT
4233771TTCCCCCCC
779389CTTTTTTTTL→PEC958_0846476Putative glycosyl hydrolase
822709GAAAAAAAASynEC958_0888281bioF8-Amino-7-oxononanoate synthase
2406295GAAAAAAAA
3892567AGGGGGGGGS→FEC958_385669ftsXCell division membrane protein
3941887GAAAAAAAASynEC958_390672chuWPutative oxygen-independent coproporphyrinogen III oxidase
4631378CTTTTTTTTK→EEC958_4497567plsBGlycerol-3-phosphate acyltransferase
  • a Boldface indicates clade B specific, and italic indicates clade C specific.

  • b Impact of SNP in bold relative to ST131 clade C2 strain EC958. Syn, synonymous change; non-synonymous changes to protein-coding genes are shown with single letter amino acid codes (EC958 sequence on left, SNP impact on right); blank lines indicate SNP in intergenic region.