TABLE 3 

Nitrogen fixation genes present in draft genomes (from metagenome data) from produced water samples collected from oil reservoirs in the Schrader Bluff formation (SB1 and SB2), the Kuparuk formation (K3), or the Ivishak Formation (I2) of the Alaska North Slopea

GenomeSampleNitrogen fixation gene(s)Affiliation
Methanosaeta_harundinacea_57_489SB1nifH (nitrogenase Fe alpha subunit), nifK (MoFe beta subunit), nifE (nitrogenase MoFe cofactor biosynthesis protein)Methanogenic Archaea
Methanocalculus_52_23SB1nifH, nifKMethanogenic Archaea
Methanoculleus_marisnigri_60_61_partialSB1nifH, nifKMethanogenic Archaea
Desulfotomaculum_46_80SB1nifH, nifK, nifE, nifB (nitrogenase cofactor biosynthesis protein)Bacteria
Methanobacteriales_53_19_partialSB2nifH, nifK,Methanogenic Archaea
Methanoculleus_60_29SB2nifH, nifK, nifE, anfO (nitrogenase iron-iron accessory protein)Methanogenic Archaea
Methanosaeta_haundinacea_56_747SB2nifHMethanogenic Archaea
Clostridia_45_118_partialSB2nifH, nifK, nifE, nifBBacteria
Methanothermobacter_50_10I2nifH, nifKMethanogenic Archaea
Methanobacteriaceae_41_258_partialI2nifH, nifKMethanogenic Archaea
Methanobacteria_50_154K3nifH, nifK, nifE, glnB (nitrogen metabolism regulatory protein)Methanogenic Archaea
  • a Genome names have the format taxonomy_percent G+C content_calculated genome coverage.