TABLE 1 

LC-MS/MS analysis of protein in C. neoformans cap59 extracts

CNAG designationDescriptionAbundanceaSignal peptidebNo. of RNA-Seq readscHomologyd
CNAG_07442Phosphatidylglycerol transfer protein100Yes25,6230.39
CNAG_01019Superoxide dismutase [Cu-Zn]97No70,7715E−62
CNAG_06432Acetate kinase50No18,367None
CNAG_00581Saccharopepsin27Yes97,1062E−107
CNAG_01230Chitin deacetylase 2/ MP9824Yes5,835None
CNAG_04625Cerevisin23Yes115,4123E−27
CNAG_00919Carboxypeptidase D19Yes9346E−19
CNAG_004503-Isopropylmalate dehydrogenase16No1,1299E−30
CNAG_00734Dihydroorotase, homodimeric type16No1,8141.5
CNAG_03232Lactamase14No23,8853E−04
CNAG_05097YjeF family protein13No8,6991E−72
CNAG_01896Alcohol dehydrogenase (NADP)11No87,4132E−71
CNAG_00264Nuclease I11Yes51,905None
CNAG_01348Cyanate hydratase9No8,7742.2
CNAG_04981Catalase7No101,1332E−102
CNAG_03223Hypothetical protein6Yes48,826None
CNAG_00575Catalase6No21,2023E−81
CNAG_01498Arylsulfatase6Yes2,9383E−43
CNAG_01137Aconitate hydratase6No19,6540
CNAG_04443Hypothetical protein5No19,4188E−10
CNAG_04269Leucyl aminopeptidase5Yes15,2970.62
CNAG_07745Alcohol dehydrogenase, propanol-preferring5No46,7343E−12
  • a The relative abundance of protein, averaged for two cap59 extracts. The most abundant protein, phosphatidylglycerol transfer protein, was arbitrarily set at 100.

  • b Determined using SignalP 4.0.

  • c Average numbers of normalized RNA-Seq reads recorded for two strains of Cryptococcus isolated directly from infected human patients (GEO accession number GSE51573) (41).

  • d Homology to the human protein with the highest similarity (BLASTp analysis); the smaller the value, the greater the similarity.