TABLE 3 

Major proteins identified in R. bromii L2-63a

R. bromii protein detectedProtein length (aa)Cell pellet bit score (% coverage)Culture supernatant bit score (% coverage)Protein ID
Glycosidases
    Amy 41,356759 (13)b1,591 (25)CBL15040.1
    Amy 1804409 (11)CBL14833.1
    Amy 27515101,150CBL14887.1
    Amy 91,056250 (4)1,135 (21)CBL16180.1
Pullulanases, type I
    Amy 101,2331,190 (19)CBL15393.1
    Amy 121,059552 (9)CBL15610.1
Hypothetical548113CBL14834.1
Pyruvate, phosphate dikinase8751,201 (28)1,291 (29)CBL14812.1
Hypothetical630389 (18)449 (16)CBL14592.1
Dockerin type I repeat73463CBL15687.1
Chaperone (DnaK)71896 (4)270 (10)CBL15021.1
Archaea/vacuole-type H+-ATPase subunit A584788 (25)CBL15964.1
Chaperonin GroL542308 (11)CBL15709.1
Aconitase643257 (9)782 (27)CBL15613.1
Putative uncharacterized572211 (11)CBL14592.1
Hypothetical1,4951,247 (19)CBL15066.1
Carbamoyl-phosphate synthase large subunit1,350187 (4)CBL14589.1
Pyruvate-flavodoxin oxidoreductase1,186162 (3)WP_021883784.1
Translocase subunit SecA955564 (14)CBL15065.1
Isoleucyl-tRNA synthetase921337CBL15771.1
Acetaldehyde dehydrogenase/alcohol dehydrogenase AdhE873640 (16)CBL14797.1
  • a Cultures were grown on RUM medium containing 0.2% soluble starch for 48 h. Proteomic analysis and the preparation of cell pellet and culture supernatants are described in Materials and Methods. Data presented are based on analysis of the most prominent spots (molecular mass, >60 kDa) recovered from two SDS gel separations of cell pellet (23 spots) and supernatant (23 spots) preparations. aa, amino acid; ID, identifier.

  • b The scores and percent coverage values shown represent the top hits against an R. bromii-encoded protein for a given protein spot in the cell pellet or supernatant fractions. Several of these proteins were detected in multiple spots recovered from 2D gels, reflecting charge variants.