TABLE 5 

Genome mapping and coverage in VirCapSeq-VERT multiplex assays

Virus7-plex mixa21-plex mix
Load (copies)%genome mappedAvg coverageLoad (copies)% genome mappedAvg coverageLoad (copies)% genome mappedAvg coverageLoad (copies)% genome mappedAvg coverage
HHV-11041004,25810499.658310699.95,43810284.510
MERS-CoV10427.91.110420.10.3410698.7231020.30
WNV10498.84,78510498.92511081007,79910299.1107
EBOV10498.93,01910497.964310599.95,01010283.67
EV-D6810499.96,64410499.84,81610699.96,91110291.864
CVV-S1041006,1971041002,3641071007,33210299.849
CVV-M1041007,6031041001,0481071007,79810210023
CVV-L1041002,4091041002421071007,73510293.44
FLUAV -11041007,8181041007,6331051007,892102100238
FLUAV -21041007,9041041007,7411051007,902102100575
FLUAV -31041007,7921041007,6581051007,906102100276
FLUAV -41041007,8001041007,5841051007,799102100594
FLUAV -51041007,7471041007,6051051007,746102100352
FLUAV -61041007,7211041007,5601051007,721102100358
FLUAV -71041007,3551041007,1001051007,711102100251
FLUAV -81041007,3671041007,3601051007,367102100397
  • a qPCR quantitated nucleic acid extracts representing seven different viruses were used to spike a background of human blood nucleic acid at levels of approximately 104 copies/100 ng, 102 copies/100 ng, and 105 to 108 copies/100 ng. Individual sequence libraries were prepared using 21 different indexes for bar coding. Libraries were mixed for capture hybridization into a 7-plex mix (libraries prepared from 104 loads) and the complete 21-plex mix.